Analysis of Salmonella typhimurium hisD3052 revertants: The use of oligodeoxyribonucleotide colony hybridization, PCR, and direct sequencing in mutational analysis
Journal Article
·
· Environmental and Molecular Mutagenesis; (United States)
- Food and Drug Administration, Washington, DC (United States)
A rapid method for determining the DNA sequences of Salmonella typhimurium hisD3052 revertants is presented. DNA colony hybridization was used to analyze revertants previously studied by Isono and Yourno. Synthetic oligodeoxyribonucleotide probes (18-mers) were able to distinguish sequences that differed by a single base pair. Mutant his sequences not identified by probing analysis were amplified using polymerase chain reaction (PCR) and directly sequenced. The combined use of DNA-colony hybridization and direct sequencing offers a precise and rapid means for the molecular characterization of hisD3052 revertants.
- OSTI ID:
- 5610674
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 18:4; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
OLIGONUCLEOTIDES
DNA SEQUENCING
REVERTANTS
DNA HYBRIDIZATION
ATP
MUTAGEN SCREENING
PHOSPHORUS 32
SALMONELLA TYPHIMURIUM
SULFUR 35
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MUTANTS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SALMONELLA
SCREENING
STRUCTURAL CHEMICAL ANALYSIS
SULFUR ISOTOPES
560300* - Chemicals Metabolism & Toxicology
OLIGONUCLEOTIDES
DNA SEQUENCING
REVERTANTS
DNA HYBRIDIZATION
ATP
MUTAGEN SCREENING
PHOSPHORUS 32
SALMONELLA TYPHIMURIUM
SULFUR 35
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MUTANTS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SALMONELLA
SCREENING
STRUCTURAL CHEMICAL ANALYSIS
SULFUR ISOTOPES
560300* - Chemicals Metabolism & Toxicology