Activated neutrophils disrupt endothelial monolayer integrity by an oxygen radical-independent mechanism

Journal Article · Fri Feb 01 00:00:00 EST 1985 · Lab. Invest.; (United States)

OSTI ID:5607930

Harlan, J M; Schwartz, B R; Reidy, M A; Schwartz, S M; Ochs, H D; Harker, L A

The effect of activated neutrophils on endothelial monolayer integrity in vitro has been measured by assessing the capacity of endothelial monolayers on polycarbonate filters to exclude /sup 125/I-albumin. Although formylmethionyl-leucyl-phenylalanine (FMLP)-activated neutrophils failed to induce /sup 51/Cr-release or detachment after 4 hours of incubation with endothelial monolayers cultured in polystyrene wells, FMLP-activated neutrophils produced a marked increase in the passage of /sup 125/I-albumin across bovine aortic or pulmonary artery endothelial monolayers on polycarbonate filters. This effect was evident as early as 30 minutes following the addition of FMLP-activated neutrophils to the monolayer and reached 180% over control values at 2 hours (p . 0.001). Light and transmission electron microscopic examination of the polycarbonate filters exposed to FMLP-activated neutrophils revealed focal disruption of the endothelial monolayers. Chronic granulomatous disease neutrophils produced similar disruption of the endothelial monolayer at 2 hours. Moreover, catalase and superoxide dismutase failed to reduce significantly the neutrophil-mediated increase in /sup 125/I-albumin passage at 2 hours. Cell-free postsecretory supernatants of FMLP-activated neutrophils, leukotriene C4, and platelet activating factor did not induce a significant increase in /sup 125/I-albumin passage across the endothelial monolayers. Of note, FMLP-activated neutrophils from a patient with a congenital abnormality of neutrophil adhesion and chemotaxis did not induce disruption of the monolayer or increase /sup 125/I-albumin passage.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 5607930
• Journal Information: Lab. Invest.; (United States), Vol. 52:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDOTHELIUM
PERMEABILITY
NEUTROPHILS
ADHESION
ALBUMINS
ARTERIES
CATALASE
CATTLE
CHROMIUM 51
ELECTRON MICROSCOPY
IODINE 125
LABELLED COMPOUNDS
OXYGEN
PHENYLALANINE
SUPEROXIDE DISMUTASE
TRACER TECHNIQUES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYMES
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MICROSCOPY
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PORPHYRINS
PROTEINS
RADIOISOTOPES
RUMINANTS
TISSUES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques