Assignment of two mitochondrially synthesized polypeptides to human mitochondrial DNA and their use in the study of intracellular mitochondrial interaction
Two mitochondrially synthesized marker polypeptides, MV-1 and MV-2, were found in human HeLa and HT1080 cells. These were assigned to the mitochondrial DNA in HeLa-HT1080 hybrids and hybrids by demonstrating their linkage to cytoplasmic genetic markers. These markers include mitochondrial DNA restriction site polymorphisms and resistance to chloramphenicol, an inhibitor of mitochondrial protein synthesis. In the absence of chloramphenicol, the expression of MV-1 and MV-2 in hybrids and hybrids was found to be directly proportional to the ratio of the parental mitochondrial DNAs. In the presence of chloramphenicol, the marker polypeptide linked to the chloramphenicol-sensitive mitochondrial DNA continued to be expressed. This demonstrated that resistant and sensitive mitochondrial DNAs can cooperate within a cell for gene expression and that the CAP-resistant allele was dominant or codominant to sensitive. Such cooperation suggests that mitochondrial DNAs can be exchanged between mitochondria.
- Research Organization:
- Dept. of Genetics, School of Medicine, Stanford Univ., Stanford, CA 94305
- OSTI ID:
- 5606786
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 2:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
CROSSING-OVER
SENSITIVITY
MITOCHONDRIA
ENZYME INDUCTION
GENE REPRESSORS
GENES
PHYSIOLOGY
POLYPEPTIDES
BIOSYNTHESIS
CHLORAMPHENICOL
HELA CELLS
HYBRIDOMAS
MAN
MOLECULAR BIOLOGY
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CELL CONSTITUENTS
DRUGS
GENE REGULATION
MAMMALS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANOIDS
PEPTIDES
PRIMATES
PROTEINS
SYNTHESIS
VERTEBRATES
550400* - Genetics
550200 - Biochemistry