Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product
- Rockefeller Univ., New York, NY (United States)
The aberrant overexpression of interleukin 6 (IL-6) is implicated as an autocrine mechanism in the enhanced proliferation of the neoplastic cell elements in various B- and T-cell malignancies and in some carcinomas and sarcomas; many of these neoplasms have been shown to be associated with a mutated p53 gene. The possibility that wild-type (wt) p53, a nuclear tumor-suppressor protein, but not its transforming mutants might serve to repress IL-6 gene expression was investigated in HeLa cells. The authors transiently cotransfected these cells with constitutive cytomegalovirus (CMV) enhancer/promoter expression plasmids overproducing wt or mutant human or murine p53 and with appropriate chloramphenicol acetyltransferase (CAT) reporter plasmids containing the promoter elements of human IL-6, c-fos, or {beta}-actin genes or of porcine major histocompatibility complex (MHC) class I gene in pN-38 to evaluate the effect of the various p53 species on these promoters. These observations identify transcriptional repression as a property of p53 and suggest that p53 and RB may be involved as transcriptional repressors in modulating IL-6 gene expression during cellular differentiation and oncogenesis.
- OSTI ID:
- 5604146
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:17; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENE REPRESSORS
TRANSCRIPTION
LYMPHOKINES
GENE REGULATION
ACTIN
CARCINOGENESIS
CELL DIFFERENTIATION
HELA CELLS
HISTOCOMPATIBILITY COMPLEX
MYCELIUM
SULFUR 35
TUMOR CELLS
ANIMAL CELLS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH FACTORS
ISOTOPES
LIGHT NUCLEI
MITOGENS
NUCLEI
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PATHOGENESIS
PLANT TISSUES
PROTEINS
RADIOISOTOPES
SULFUR ISOTOPES
TISSUES
550200* - Biochemistry