Plasma membrane associated, virus-specific polypeptides required for the formation of target antigen complexes recognized by virus-specific cytotoxic T lymphocytes

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:5601408

Domber, E A

These studies were undertaken to define some of the poxvirus-specific target antigens which are synthesized in infected cells and recognized by vaccinia virus-specific CTLs (VV-CTLs). Since vaccinia virus infected, unmanipulated target cells express numerous virus-specific antigens on the plasma membrane, attempts were made to manipulate expression of the poxvirus genome after infection so that one or a few defined virus-specified antigens were expressed on the surface of infected cells. In vitro (/sup 51/Cr)-release assays determined that viral DNA synthesis and expression of late viral proteins were not necessary to form a target cell which was fully competent for lysis by VV-CTLs. Under the conditions employed in these experiments, 90-120 minutes of viral protein synthesis were necessary to produce a competent cell for lysis by VV-CTLs. In order to further inhibit the expression of early viral proteins in infected cells, partially UV-inactivated vaccinia virus was employed to infect target cells. It was determined that L-cells infected with virus preparations which had been UV-irradiated for 90 seconds were fully competent for lysis by VV-CTLs. Cells infected with 90 second UV-irr virus expressed 3 predominant, plasma membrane associated antigens of 36-37K, 27-28K, and 19-17K. These 3 viral antigens represent the predominant membrane-associated viral antigens available for interaction with class I, major histocompatibility antigens and hence are potential target antigens for VV-CTLs.

• Research Organization: Rutgers-the State Univ., New Brunswick, NJ (USA)
• OSTI ID: 5601408
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMIUM 51
MEMBRANE TRANSPORT
VACCINIA VIRUS
BIOLOGICAL RADIATION EFFECTS
IMMUNOLOGY
ANTIGENS
BIOSYNTHESIS
CELL MEMBRANES
DNA REPLICATION
IN VITRO
LYMPHOCYTES
POLYPEPTIDES
PROTEINS
ULTRAVIOLET RADIATION
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
MATERIALS
MEMBRANES
MICROORGANISMS
NUCLEI
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SOMATIC CELLS
SYNTHESIS
VIRUSES
550201* - Biochemistry- Tracer Techniques