Viability and functional integrity of washed platelets

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Transfusion (Philadelphia); (United States)

DOI:https://doi.org/10.1046/j.1537-2995.1989.29689318452.x· OSTI ID:5601382

Pineda, A A; Zylstra, V W; Clare, D E; Dewanjee, M K; Forstrom, L A

The viability and functional integrity of saline- and ACD-saline-washed platelets were compared with those of unwashed platelets. After template bleeding time (TBT) was measured, 15 healthy volunteers underwent plateletpheresis and ingested 600 mg of aspirin. Autologous /sup 111/In-labeled platelets were transfused: unwashed (n = 5), washed with 0.9 percent saline solution (SS) (n = 5), and washed with a buffered 12.6 percent solution of ACD-A in 0.9 percent saline solution (n = 5). After transfusion, we measured TBT at 1, 4, and 24 hours; platelet survival at 10 minutes and 1, 4, and 24 hours and daily for 6 days; and the percentage of uptake in liver and spleen by quantitative whole-body radionuclide scintigraphy at 24 and 190 hours. We found that saline washing affected platelet recovery, 23.47 +/- 12 percent (p less than 0.001) as compared to 52.43 +/- 17 percent (p less than 0.002) for ACD-saline and 73.17 +/- 8 percent for control; that saline washing resulted in a greater liver uptake than control and ACD-saline-washed platelets (31.9 +/- 8% (p less than 0.001) vs 17.7 +/- 4.1 and 19.3 +/- 2.1% (p greater than 0.1), respectively); that, unlike control and ACD-saline-washed platelets, saline-washed platelets did not shorten bleeding time; and that neither type of washing affected survival. Although ACD-saline washing affects recovery, it also results in intact function, normal survival, higher recovery than SS platelets, and no significant liver uptake.

• Research Organization: Mayo Clinic, Rochester, MN (USA)
• OSTI ID: 5601382
• Journal Information: Transfusion (Philadelphia); (United States), Vol. 29:6
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD PLATELETS
VIABILITY
LIVER
SCINTISCANNING
SODIUM CHLORIDES
BIOLOGICAL EFFECTS
SPLEEN
BIOLOGICAL RECOVERY
BUFFERS
GLUCOSE
INDIUM 111
MAN
SURVIVAL TIME
TRANSFUSIONS
UPTAKE
WHOLE-BODY COUNTING
ALDEHYDES
ALKALI METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CHLORIDES
CHLORINE COMPOUNDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HALIDES
HALOGEN COMPOUNDS
HEXOSES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RADIOISOTOPE SCANNING
RADIOISOTOPES
RECOVERY
SACCHARIDES
SODIUM COMPOUNDS
THERAPY
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics