Transport and metabolism of a sucrose analog (1'-fluorosucrose) into Zea mays L. Endosperm without invertase hydrolysis

Journal Article · Tue Dec 01 00:00:00 EST 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.85.4.902· OSTI ID:5600485

Schmalstig, J G; Hitz, W D

1'-fluorosucrose (FS), a sucrose analog resistant to hydrolysis by invertase, was transported from husk leaves into maize (Zea mays L.) kernels with the same magnitude and kinetics as sucrose. /sup 14/C-Label from (/sup 14/C)FS and (/sup 14/C)sucrose in separate experiments was distributed similarly between the pedicel, endosperm, and embryo with time. FS passed through maternal tissue and was adsorbed intact into the endosperm where it was metabolized and used in synthesis of sucrose and methanol-chloroform-water insolubles. Accumulation of (/sup 14/C)sucrose from supplied (/sup 14/C)glucosyl-FS indicated that the glucose moiety from the breakdown of sucrose (here FS), which normally occurs in the process of starch synthesis in maize endosperm, was available to the pool of substrates for resynthesis of sucrose. Uptake of FS into maize endosperm without hydrolysis suggest that despite the presence of invertase in maternal tissues and the hydrolysis of a large percentage of sucrose unloaded form the phloem, hexoses are not specifically needed for uptake into maize endosperm.

• Research Organization: E. I. DuPont de Nemours and Company, Wilmington, DE
• OSTI ID: 5600485
• Journal Information: Plant Physiol.; (United States), Vol. 85:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ISOMERASES
ENZYME ACTIVITY
SACCHAROSE
MEMBRANE TRANSPORT
METABOLISM
CARBON 14 COMPOUNDS
ENZYMATIC HYDROLYSIS
MAIZE
TRACER TECHNIQUES
CARBOHYDRATES
CEREALS
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMES
GRASS
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SOLVOLYSIS
550501* - Metabolism- Tracer Techniques