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Title: Antiviral T cell competence and restriction specificity of mixed allogeneic (P1 + P2----P1) irradiation chimeras

Abstract

Mixed irradiation bone marrow chimeras were prepared by reconstituting lethally irradiated C57BL/10 (B10) or B10.D2 mice with T cell-depleted bone marrow cells of B10 plus B10.D2 origin. These chimeras were healthy and survived well under conventional housing conditions and after experimental laboratory infections. Of a total of 17 chimeras tested, 2 died spontaneously or from the injected virus. Twelve of fifteen chimeras mounted a measurable cytotoxic T cell response to virus. Despite approximately equal percentages of B10 and B10.D2 lymphocytes in chimeras, cytotoxic T cell responses to vaccinia virus and lymphocytic choriomeningitis virus were mediated variably by either syngeneic or allogeneic donor lymphocytes; thus the H-2 type of effector T cells frequently did not correspond to the 50:50 distribution of spleen or peripheral blood lymphocytes. Cytotoxic responses were restricted exclusively to recipient H-2 type. All mixed chimeras examined were able to mount a good IgG response to vesicular stomatitis virus. These results confirm previous data suggesting that such mixed chimeras are healthy and immunocompetent and demonstrate strict recipient-determined restriction specificity of effector T cells; they also suggest that if T help is necessary for induction of virus-specific cytotoxic T cells, it does not require host-restricted interactions between helper T cellsmore » and precursor cytotoxic T cells.« less

Authors:
; ; ; ; ; ; ;  [1]
  1. National Cancer Institute, Bethesda, MD (USA)
Publication Date:
OSTI Identifier:
5599911
Resource Type:
Journal Article
Journal Name:
Cellular Immunology; (USA)
Additional Journal Information:
Journal Volume: 121:1; Journal ID: ISSN 0008-8749
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOCYTES; SPECIFICITY; RADIATION CHIMERAS; IMMUNE REACTIONS; VIRUSES; INFECTIVITY; ANTIGENS; BONE MARROW; IMMUNOGLOBULINS; LETHAL IRRADIATION; MICE; TOXICITY; VIRAL DISEASES; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHIMERAS; CONNECTIVE TISSUE CELLS; DISEASES; GLOBULINS; HEMATOPOIETIC SYSTEM; INFECTIOUS DISEASES; IRRADIATION; LEUKOCYTES; MAMMALS; MATERIALS; MICROORGANISMS; MOSAICISM; ORGANIC COMPOUNDS; ORGANS; PARASITES; PROTEINS; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Rueedi, E S, Sykes, M, Ildstad, S T, Chester, C H, Althage, A, Hengartner, H, Sachs, D H, and Zinkernagel, R M. Antiviral T cell competence and restriction specificity of mixed allogeneic (P1 + P2----P1) irradiation chimeras. United States: N. p., 1989. Web. doi:10.1016/0008-8749(89)90016-6.
Rueedi, E S, Sykes, M, Ildstad, S T, Chester, C H, Althage, A, Hengartner, H, Sachs, D H, & Zinkernagel, R M. Antiviral T cell competence and restriction specificity of mixed allogeneic (P1 + P2----P1) irradiation chimeras. United States. https://doi.org/10.1016/0008-8749(89)90016-6
Rueedi, E S, Sykes, M, Ildstad, S T, Chester, C H, Althage, A, Hengartner, H, Sachs, D H, and Zinkernagel, R M. 1989. "Antiviral T cell competence and restriction specificity of mixed allogeneic (P1 + P2----P1) irradiation chimeras". United States. https://doi.org/10.1016/0008-8749(89)90016-6.
@article{osti_5599911,
title = {Antiviral T cell competence and restriction specificity of mixed allogeneic (P1 + P2----P1) irradiation chimeras},
author = {Rueedi, E S and Sykes, M and Ildstad, S T and Chester, C H and Althage, A and Hengartner, H and Sachs, D H and Zinkernagel, R M},
abstractNote = {Mixed irradiation bone marrow chimeras were prepared by reconstituting lethally irradiated C57BL/10 (B10) or B10.D2 mice with T cell-depleted bone marrow cells of B10 plus B10.D2 origin. These chimeras were healthy and survived well under conventional housing conditions and after experimental laboratory infections. Of a total of 17 chimeras tested, 2 died spontaneously or from the injected virus. Twelve of fifteen chimeras mounted a measurable cytotoxic T cell response to virus. Despite approximately equal percentages of B10 and B10.D2 lymphocytes in chimeras, cytotoxic T cell responses to vaccinia virus and lymphocytic choriomeningitis virus were mediated variably by either syngeneic or allogeneic donor lymphocytes; thus the H-2 type of effector T cells frequently did not correspond to the 50:50 distribution of spleen or peripheral blood lymphocytes. Cytotoxic responses were restricted exclusively to recipient H-2 type. All mixed chimeras examined were able to mount a good IgG response to vesicular stomatitis virus. These results confirm previous data suggesting that such mixed chimeras are healthy and immunocompetent and demonstrate strict recipient-determined restriction specificity of effector T cells; they also suggest that if T help is necessary for induction of virus-specific cytotoxic T cells, it does not require host-restricted interactions between helper T cells and precursor cytotoxic T cells.},
doi = {10.1016/0008-8749(89)90016-6},
url = {https://www.osti.gov/biblio/5599911}, journal = {Cellular Immunology; (USA)},
issn = {0008-8749},
number = ,
volume = 121:1,
place = {United States},
year = {Thu Jun 01 00:00:00 EDT 1989},
month = {Thu Jun 01 00:00:00 EDT 1989}
}