Characterization of hemoglobin adduct formation in mice and rats after administration of ( sup 14 C)butadiene or ( sup 14 C)isoprene

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Toxicology and Applied Pharmacology; (USA)

DOI:https://doi.org/10.1016/0041-008X(89)90093-8· OSTI ID:5599388

Sun, J D; Dahl, A R; Bond, J A; Birnbaum, L S; Henderson, R F ^[1]

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1. Lovelace Biomedical and Environmental Research Institute, Albuquerque, NM (USA)

Occupational exposures to 1,3-butadiene or isoprene occur through their use in the manufacture of rubber and other related polymer products. The purpose of this study was to determine if butadiene or isoprene administration would result in the formation of adducts with blood hemoglobin (Hb), and if such adducts can be used as a measure of previous exposure(s). Male B6C3F1 mice and male Sprague-Dawley rats were injected intraperitoneally with 1, 10, 100, or 1000 mumol (14C)butadiene or 0.3, 3.0, 300, 1000, or 3000 mumol (14C)isoprene per kilogram body weight. Animals were killed 24 hr later. Globin was isolated from blood samples and was analyzed for 14C by liquid scintillation spectroscopy. Hb adduct formation was linearly related to administered doses up to 100 mumol (14C)butadiene or 500 mumol (14C)isoprene per kilogram body weight for mice and rats, respectively. For (14C)butadiene, the efficiency of Hb adduct formation in mice and rats within the linear response range was 0.177 +/- 0.003 and 0.407 +/- 0.019 (pmol of 14C-adducts/mg globin)/(mumol of retained (14C)butadiene/kg body wt), respectively (mean +/- SE; n = 18). For (14C)isoprene, these values for mice and rats were 0.158 +/- 0.035 and 0.079 +/- 0.016 (pmol of 14C-adducts/mg globin)/(mumol of retained (14C)isoprene/kg body wt), respectively (mean +/- SE; n = 12). Hb adducts also accumulated linearly after repeated daily administration of 100 mumol (14C)butadiene or 500 mumol (14C)isoprene per kilogram body wt to mice and rats, respectively, for 3 days. (14C)Butadiene-derived Hb adducts in blood showed lifetimes of approximately 24 and approximately 65 days for mice and rats, respectively, which correlate with the reported lifetimes for red blood cells in these rodent species. Thus, levels of butadiene- or isoprene-derived adducts on Hb in circulating blood may be a useful measure of prior repeated exposures to these compounds.

• OSTI ID: 5599388
• Journal Information: Toxicology and Applied Pharmacology; (USA), Vol. 100:1; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BUTADIENE
TOXICITY
DNA ADDUCTS
BIOSYNTHESIS
ISOPRENE
CARBON 14 COMPOUNDS
CHEMICAL COMPOSITION
HEMOGLOBIN
MICE
OCCUPATIONAL EXPOSURE
RATS
TRACER TECHNIQUES
ADDUCTS
ANIMALS
CARBOXYLIC ACIDS
DIENES
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
POLYENES
PORPHYRINS
PROTEINS
RODENTS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques