Mutants of thermotaxis in Dictyostelium discoideum
Amoebae of Dictyostelium discoideum, strain HL50 were mutagenized with N-methyl-N'-nitro-N-nitrosoguanidine, cloned, allowed to form pseudoplasmodia and screened for aberrant positive and negative thermotaxis. Three types of mutants were found. Mutant HO428 exhibits only positive thermotaxis over the entire temperature range (no negative thermotaxis). HO596 and HO813 exhibit weakened positive thermotaxis and normal negative thermotaxis. The weakened positive thermotactic response results in a shift toward warmer temperatures in the transition temperature from negative to positive thermotaxis. Mutant HO209 exhibits weakened positive and negative thermotactic responses and has a transition temperature similar to the 'wild type' (HL50).The two types of mutants represented by HO428, HO596 and HO813 support the model that positive and negative thermotaxis have separate pathways for temperature sensing. The type of mutants which contains HO209 suggests that those two pathways converge at some point before the response.
- Research Organization:
- Michigan State Univ., East Lansing
- DOE Contract Number:
- AC02-76ER01338
- OSTI ID:
- 5596169
- Journal Information:
- Exp. Cell Res.; (United States), Vol. 140:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMOEBA
MUTANTS
TEMPERATURE EFFECTS
BIOLOGICAL PATHWAYS
NITROSO COMPOUNDS
ANIMALS
INVERTEBRATES
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTOZOA
SARCODINA
560202* - Thermal Effects- Microorganisms- (1987)