Starch and sucrose synthesis in Phaseolus vulgaris as affected by light, CO/sub 2/, and abscisic acid
Phaseolus vulgaris L. leaves were subjected to various light, CO/sub 2/, and O/sub 2/ levels and abscisic acid, then given a 10 minute pulse of /sup 14/CO/sub 2/ followed by a 5 minute chase with unlabeled CO/sub 2/. After the chase period, very little label remained in the ionic fractions except at low CO/sub 2/ partial pressure. Most label was found in the neutral, alcohol soluble fraction or in the insoluble fraction digestable by amyloglucosidase. Sucrose formation was linearly related to assimilation rate. Starch formation increased linearly with assimilation rate, but did not occur if the assimilation rate was below 4 micromoles per square meter per second. Neither abscisic acid, nor high CO/sub 2/ in combination with low O/sub 2/ caused significant perturbations of the sucrose/starch formation ratio. These studies indicate that the pathways for starch and sucrose synthesis both are controlled by the rate of net CO/sub 2/ assimilation, with sucrose the preferred product at very low assimilation rates.
- Research Organization:
- Desert Research Institute, Reno, NV
- OSTI ID:
- 5591335
- Journal Information:
- Plant Physiol.; (United States), Vol. 77:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ABSCISIC ACID
BIOLOGICAL EFFECTS
CARBON DIOXIDE
SACCHAROSE
BIOSYNTHESIS
STARCH
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
LEAVES
PHASEOLUS
PHOTOSYNTHESIS
VISIBLE RADIATION
AUXINS
BACTERIA
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHEMICAL REACTIONS
DISACCHARIDES
ELECTROMAGNETIC RADIATION
LABELLED COMPOUNDS
LEGUMINOSAE
MICROORGANISMS
MONOCARBOXYLIC ACIDS
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PLANT GROWTH REGULATORS
PLANTS
POLYSACCHARIDES
RADIATIONS
REAGENTS
RHIZOBIUM
SACCHARIDES
SYNTHESIS
550501* - Metabolism- Tracer Techniques