Eimeria tenella: parasite-specific incorporation of /sup 3/H-uracil as a quantitative measure of intracellular development
An assay has been developed using parasite-specific incorporation of /sup 3/H-uracil to assess the intracellular growth of Eimeria tenella in vitro. As shown by both scintillation counts and autoradiography, /sup 3/H-uracil was incorporated specifically into intracellular parasites from the onset of infection and continued throughout development of the first generation schizonts. Mature schizonts and first generation merozoites did not continue to incorporate additional /sup 3/H-uracil, indicating that RNA synthesis had halted in these stages. Based on these findings, a semi-automated microscale uracil incorporation assay was developed to determine parasite viability. This method should be useful for biochemical studies with intracellular parasites and for screening compounds for anticoccidial activity. The ease, rapidity, and quantitative nature of this assay contrasts favorably with standard morphometric approaches of determining parasite development. In addition, parallel studies using host cell incorporation of /sup 3/H-uridine have been introduced as a method of determining whether antiparasitic activity is direct or indirect in relation to effects on the host cell.
- Research Organization:
- Merck and Co., Inc., Rahway, NJ
- OSTI ID:
- 5586757
- Journal Information:
- J. Protozool.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PARASITES
GROWTH
INFECTIVITY
AUTORADIOGRAPHY
BIOASSAY
CATTLE
RNA
SCINTILLATION COUNTING
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
URINE
VIABILITY
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
COUNTING TECHNIQUES
DOMESTIC ANIMALS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RUMINANTS
VERTEBRATES
WASTES
550901* - Pathology- Tracer Techniques