Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans
Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in hydrochloric acid. THe separation techniques also proved useful in fractionation of exoglycosidase digests of O- and N-linked oligosaccharides. Separations of aldoses, hexosamines, and uronic acids were adapted to sensitive photometric detection.
- Research Organization:
- Univ. of Lund, Sweden
- OSTI ID:
- 5585259
- Journal Information:
- Anal. Biochem.; (United States), Vol. 154:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
LIQUID COLUMN CHROMATOGRAPHY
GLUCOPROTEINS
CATIONS
FORMIC ACID
GALACTOSE
GLUCOSAMINE
GLUCOSE
GLUCURONIC ACID
HEXOSAMINES
HEXOSES
HYDROCHLORIC ACID
HYDROLYSIS
ION EXCHANGE
LABELLED COMPOUNDS
LACTONES
MANNOSE
OLIGOSACCHARIDES
PHOTOMETRY
POLYSACCHARIDES
SCINTILLATION COUNTING
SULFURIC ACID
TRACER TECHNIQUES
URONIC ACIDS
XYLOSE
ALDEHYDES
AMINES
CARBOXYLIC ACIDS
CHARGED PARTICLES
CHEMICAL REACTIONS
CHROMATOGRAPHY
COUNTING TECHNIQUES
DECOMPOSITION
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
INORGANIC ACIDS
IONS
ISOTOPE APPLICATIONS
LYSIS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PENTOSES
PROTEINS
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
400102* - Chemical & Spectral Procedures
400104 - Spectral Procedures- (-1987)
550201 - Biochemistry- Tracer Techniques