Processing and transfer of epidermal growth factor in developing rat jejunum and ileum

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Peptides (Fayetteville, New York); (USA)

DOI:https://doi.org/10.1016/0196-9781(90)90136-S· OSTI ID:5583664

Rao, R K; Koldovsky, O; Korc, M; Pollack, P F; Wright, S; Davis, T P ^[1]

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1. Univ. of Arizona College of Medicine, Tucson (USA)

Using everted sac technique we demonstrated the transfer of {sup 125}I-mEGF across the jejunal and ileal walls of suckling, weanling and adult rats. The transfer by the suckling rat jejunum and ileum was significantly inhibited by the presence of dinitrophenol and sodium azide or by the replacement of sodium with potassium or choline, RP-HPLC analysis detected carboxy-terminal processing of {sup 125}I-mEGF in suckling and adult rat jejunum and ileum. Suckling rat jejunum produced {sup 125}I-des(53)mEGF and {sup 125}I-des(49-53)mEGF, whereas {sup 125}I-des(48-53)mEGF was detected in suckling rat ileum or adult rat jejunum and ileum. All three forms of {sup 125}I-mEGF bound to anti-EGF antibody and EGF receptors. The receptor binding of {sup 125}I-des(53)mEGF was higher than that of {sup 125}I-mEGF, but those of {sup 125}I-des(49-53)mEGF and {sup 125}I-des(48-53)mEGF were greatly diminished. Results indicate a carboxy-terminal processing of mouse EGF during uptake and transfer in the small intestine of developing and adult rats, and the resulting products showed altered receptor binding. An identical amino acid sequence of the C-terminal pentapeptide of eGF from mouse, human and possibly rat may suggest a biological significance of C-terminal processing of EGF in the small intestine.

• OSTI ID: 5583664
• Journal Information: Peptides (Fayetteville, New York); (USA), Vol. 11:6; ISSN 0196-9781
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
MEMBRANE TRANSPORT
SMALL INTESTINE
CELL DIFFERENTIATION
AMINO ACID SEQUENCE
BIOCHEMICAL REACTION KINETICS
CHROMATOGRAPHY
IN VITRO
INTESTINAL ABSORPTION
IODINE 124
RATS
RECEPTORS
TRACER TECHNIQUES
ABSORPTION
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
UPTAKE
VERTEBRATES
550201* - Biochemistry- Tracer Techniques