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Title: Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme

Abstract

The potentiometric acid-base titration curves of fully protonated lysozyme at ionic strenghts of 0.10 and 1.0 M has been performed. The stoichiometry and the pK/sub a/ values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with pK/sub a/ values of 3.76 and 5.02, the imidazole group with pK/sub a/ 7.37 and the amino group with pK/sub a/ 9.63, have been identified at an ionic strength of 0.10 M at 25.0/sup 0/C. The number of titratable groups found per mole of protein has been 5.12 and 5.60 for the two types of carboxylic groups, 1.13 for the imidazole group, and 3.19 for the amino groups. The endpoint of the titration of the protein obtained by this method accords quite well with the endpoint obtained by the use of Gran function applied to the excess of strong base.

Authors:
 [1]; ;
  1. Univ. Estadual de Campinas, Brazil
Publication Date:
OSTI Identifier:
5581296
Resource Type:
Journal Article
Journal Name:
Anal. Biochem.; (United States)
Additional Journal Information:
Journal Volume: 123:2
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; LYSOZYME; CHEMICAL REACTION KINETICS; MOLECULAR STRUCTURE; AMINO ACIDS; CARBOXYLIC ACIDS; IMIDAZOLES; STOICHIOMETRY; TITRATION; AZOLES; ENZYMES; GLYCOSYL HYDROLASES; HETEROCYCLIC COMPOUNDS; HYDROLASES; KINETICS; O-GLYCOSYL HYDROLASES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; REACTION KINETICS; 400102* - Chemical & Spectral Procedures

Citation Formats

Godinho, O E.S., Aleixo, L M, and Alves, J P.H. Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme. United States: N. p., 1982. Web. doi:10.1016/0003-2697(82)90441-9.
Godinho, O E.S., Aleixo, L M, & Alves, J P.H. Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme. United States. https://doi.org/10.1016/0003-2697(82)90441-9
Godinho, O E.S., Aleixo, L M, and Alves, J P.H. 1982. "Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme". United States. https://doi.org/10.1016/0003-2697(82)90441-9.
@article{osti_5581296,
title = {Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme},
author = {Godinho, O E.S. and Aleixo, L M and Alves, J P.H.},
abstractNote = {The potentiometric acid-base titration curves of fully protonated lysozyme at ionic strenghts of 0.10 and 1.0 M has been performed. The stoichiometry and the pK/sub a/ values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with pK/sub a/ values of 3.76 and 5.02, the imidazole group with pK/sub a/ 7.37 and the amino group with pK/sub a/ 9.63, have been identified at an ionic strength of 0.10 M at 25.0/sup 0/C. The number of titratable groups found per mole of protein has been 5.12 and 5.60 for the two types of carboxylic groups, 1.13 for the imidazole group, and 3.19 for the amino groups. The endpoint of the titration of the protein obtained by this method accords quite well with the endpoint obtained by the use of Gran function applied to the excess of strong base.},
doi = {10.1016/0003-2697(82)90441-9},
url = {https://www.osti.gov/biblio/5581296}, journal = {Anal. Biochem.; (United States)},
number = ,
volume = 123:2,
place = {United States},
year = {Thu Jul 01 00:00:00 EDT 1982},
month = {Thu Jul 01 00:00:00 EDT 1982}
}