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Title: Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells

Abstract

Psoralen compounds covalently bind to nucleic acids when irradiated with long-wavelength ultraviolet light. This treatment can destroy the infectivity of deoxyribonucleic acid and ribonucleic acid viruses. Two psoralen compounds, 4'-hydroxymethyltrioxsalen and 4'-aminomethyltrioxsalen, were used with long-wavelength ultraviolet light to inactivate cell-free herpes simplex and influenza viruses and to render virus-infected cells noninfectious. This method of inactivation was compared with germicidal (short-wavelength) ultraviolet light irradiation. The antigenicity of the treated, virus-infected, antigen-bearing cells was examined by immunofluorescence and radioimmunoassay and by measuring the capacity of the herpes simplex virus-infected cells to stimulate virus-specific lymphocyte proliferation. The infectivity of the virus-infected cells could be totally eliminated without altering their viral antigenicity. The use of psoralen plus long-wavelength ultraviolet light is well suited to the preparation of noninfectious virus antigens and virus antigen-bearing cells for immunological assays.

Authors:
; ; ;
Publication Date:
OSTI Identifier:
5576958
Resource Type:
Journal Article
Journal Name:
Infect. Immun.; (United States)
Additional Journal Information:
Journal Volume: 32:3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; ANTIGENS; CHEMICAL PREPARATION; RADIOIMMUNOASSAY; HERPES SIMPLEX; INACTIVATION; RADIOINDUCTION; INFLUENZA VIRUSES; PSORALEN; BIOLOGICAL EFFECTS; CELL PROLIFERATION; FLUORESCENCE; LYMPHOCYTES; NEAR ULTRAVIOLET RADIATION; ANIMAL CELLS; ANTICOAGULANTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; COUMARINS; DISEASES; DRUGS; ELECTROMAGNETIC RADIATION; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; LEUKOCYTES; LUMINESCENCE; MATERIALS; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PARASITES; RADIATIONS; RADIOASSAY; SKIN DISEASES; SOMATIC CELLS; SYNTHESIS; TRACER TECHNIQUES; ULTRAVIOLET RADIATION; VIRAL DISEASES; VIRUSES; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987); 550700 - Microbiology

Citation Formats

Redfield, D C, Richman, D D, Oxman, M N, and Kronenberg, L H. Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells. United States: N. p., 1981. Web.
Redfield, D C, Richman, D D, Oxman, M N, & Kronenberg, L H. Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells. United States.
Redfield, D C, Richman, D D, Oxman, M N, and Kronenberg, L H. 1981. "Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells". United States.
@article{osti_5576958,
title = {Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells},
author = {Redfield, D C and Richman, D D and Oxman, M N and Kronenberg, L H},
abstractNote = {Psoralen compounds covalently bind to nucleic acids when irradiated with long-wavelength ultraviolet light. This treatment can destroy the infectivity of deoxyribonucleic acid and ribonucleic acid viruses. Two psoralen compounds, 4'-hydroxymethyltrioxsalen and 4'-aminomethyltrioxsalen, were used with long-wavelength ultraviolet light to inactivate cell-free herpes simplex and influenza viruses and to render virus-infected cells noninfectious. This method of inactivation was compared with germicidal (short-wavelength) ultraviolet light irradiation. The antigenicity of the treated, virus-infected, antigen-bearing cells was examined by immunofluorescence and radioimmunoassay and by measuring the capacity of the herpes simplex virus-infected cells to stimulate virus-specific lymphocyte proliferation. The infectivity of the virus-infected cells could be totally eliminated without altering their viral antigenicity. The use of psoralen plus long-wavelength ultraviolet light is well suited to the preparation of noninfectious virus antigens and virus antigen-bearing cells for immunological assays.},
doi = {},
url = {https://www.osti.gov/biblio/5576958}, journal = {Infect. Immun.; (United States)},
number = ,
volume = 32:3,
place = {United States},
year = {Mon Jun 01 00:00:00 EDT 1981},
month = {Mon Jun 01 00:00:00 EDT 1981}
}