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Title: Sulfur uptake by type I collagen from methyl mercaptan/dimethyl disulfide air mixtures

Abstract

Type I acid-soluble collagen, suspended in 0.02 M Tris/0.13 M NaCl buffer (pH 7.4), was exposed to air atmosphere admixed with 10.7 X 10(-9) moles of (35S)-labeled dimethyl disulphide/methyl mercaptan mixture in the ratio of 99.82% (CH3S)2/0.18% CH3SH. Gas chromatographic analyses of head-space following one and four days of incubation indicated that all of the CH3SH was absorbed from the head-space by the collagen-containing liquid phase, while the (CH3S)2 concentration of the head-space remained essentially unchanged. Of the total (35S)-activity initially present in the head-space, only 1.0 the total (35S)-activity initially present in the head-space, only 1.0 and 1.86% was absorbed by the collagen-containing liquid phase within one and four days, respectively. Paper chromatographic separation of the reaction mixture showed that only 0.42 and 0.60% of the radioactivity in the head-space was associated with collagen after one and four days of reaction. In comparable (35S)-H2S systems devoid of (CH3S)2, all of the H2S was absorbed within 48 hours of incubation with 18.04% (1 day) and 18.28% (4 days) of the available (35S)-H2S complexed with collagen. The results provide evidence that the reaction of collagen with H2S and CH3SH/(CH3S)2 mixture proceeded via the H2S and CH3SH thiol groups.

Authors:
;
Publication Date:
Research Org.:
Univ. of British Columbia, Vancouver
OSTI Identifier:
5575352
Resource Type:
Journal Article
Journal Name:
J. Dent. Res.; (United States)
Additional Journal Information:
Journal Volume: 12
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; COLLAGEN; SORPTIVE PROPERTIES; SULFUR; ADSORPTION; AIR; DISULFIDES; HYDROGEN SULFIDES; RATS; SOLVENTS; SULFUR 35; THIOLS; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CHALCOGENIDES; DAYS LIVING RADIOISOTOPES; ELEMENTS; EVEN-ODD NUCLEI; FLUIDS; GASES; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; NONMETALS; NUCLEI; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PROTEINS; RADIOISOTOPES; RODENTS; SCLEROPROTEINS; SORPTION; SULFIDES; SULFUR COMPOUNDS; SULFUR ISOTOPES; SURFACE PROPERTIES; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

Citation Formats

Johnson, P W, and Tonzetich, J. Sulfur uptake by type I collagen from methyl mercaptan/dimethyl disulfide air mixtures. United States: N. p., 1985. Web. doi:10.1177/00220345850640120601.
Johnson, P W, & Tonzetich, J. Sulfur uptake by type I collagen from methyl mercaptan/dimethyl disulfide air mixtures. United States. https://doi.org/10.1177/00220345850640120601
Johnson, P W, and Tonzetich, J. 1985. "Sulfur uptake by type I collagen from methyl mercaptan/dimethyl disulfide air mixtures". United States. https://doi.org/10.1177/00220345850640120601.
@article{osti_5575352,
title = {Sulfur uptake by type I collagen from methyl mercaptan/dimethyl disulfide air mixtures},
author = {Johnson, P W and Tonzetich, J},
abstractNote = {Type I acid-soluble collagen, suspended in 0.02 M Tris/0.13 M NaCl buffer (pH 7.4), was exposed to air atmosphere admixed with 10.7 X 10(-9) moles of (35S)-labeled dimethyl disulphide/methyl mercaptan mixture in the ratio of 99.82% (CH3S)2/0.18% CH3SH. Gas chromatographic analyses of head-space following one and four days of incubation indicated that all of the CH3SH was absorbed from the head-space by the collagen-containing liquid phase, while the (CH3S)2 concentration of the head-space remained essentially unchanged. Of the total (35S)-activity initially present in the head-space, only 1.0 the total (35S)-activity initially present in the head-space, only 1.0 and 1.86% was absorbed by the collagen-containing liquid phase within one and four days, respectively. Paper chromatographic separation of the reaction mixture showed that only 0.42 and 0.60% of the radioactivity in the head-space was associated with collagen after one and four days of reaction. In comparable (35S)-H2S systems devoid of (CH3S)2, all of the H2S was absorbed within 48 hours of incubation with 18.04% (1 day) and 18.28% (4 days) of the available (35S)-H2S complexed with collagen. The results provide evidence that the reaction of collagen with H2S and CH3SH/(CH3S)2 mixture proceeded via the H2S and CH3SH thiol groups.},
doi = {10.1177/00220345850640120601},
url = {https://www.osti.gov/biblio/5575352}, journal = {J. Dent. Res.; (United States)},
number = ,
volume = 12,
place = {United States},
year = {Sun Dec 01 00:00:00 EST 1985},
month = {Sun Dec 01 00:00:00 EST 1985}
}