Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. Characterization of a soluble suppressor of B cell immunoglobulin production
Human peripheral blood mononuclear cells (PBMC) activated with the mitogenic lectin concanavalin A (Con A) elaborate a soluble immune suppressor supernatant (SISS) that contains at least 2 distinct suppressor factors. One of these, SISS-B, inhibits polyclonal B cell immunoglobulin production, whereas the other, SISS-T, suppresses T cell proliferation to both mitogens and antigens. The latter mediator is discussed in the companion paper. Characteristics of the human soluble suppressor of B cell immunoglobulin production (SISS-B) include: 1) inhibition by a noncytotoxic mechanism, 2) loss of activity in the presence of the monosaccharide L-rhamnose, 3) appearance within 8 to 16 hr after the addition of Con A, 4) elaboration by cells irradiated with 500 or 2000 rads, 5) production by highly purified T cells, 6) stability at pH 2.5 but instability at 56/sup o/C, and 7) m.w. of 60 to 80,000. These data indicate that after Con A activation, selected T cells not only become potent suppressor cells, but also generate a soluble saccharide-specific factor(s) that inhibits polyclonal immunoglobulin production by human B cells.
- Research Organization:
- National Institutes of Health, Bethesda, MD
- OSTI ID:
- 5571695
- Journal Information:
- J. Immunol.; (United States), Vol. 126:3
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
BLOOD CELLS
IRRADIATION
IMMUNOGLOBULINS
INHIBITION
IMMUNOSUPPRESSION
CHEMICAL ACTIVATION
ANTIGENS
CELL PROLIFERATION
EXPERIMENTAL DATA
IMMUNE REACTIONS
LYMPHOCYTES
MAN
MITOGENS
MONOSACCHARIDES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DATA
GLOBULINS
INFORMATION
LEUKOCYTES
MAMMALS
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550300 - Cytology