Oxygen requirement for growth of Candida utilis on semisolid straw substrate

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260300512· OSTI ID:5564571

Han, Y W

Semisolid fermentation has been used for production of enzymes, mushrooms, fermented food and animal feed. The main difference between the submerged liquid fermentation and the semisolid cultivation is that the substrate in the former is completely dissolved and homogeneous, whereas the latter employs insoluble substrate with relatively little liquid in the growth environment. In spite of its simplicity and ease of operation, due to heterogeneity of the fermentation mixture, the controls for microbial growth in semisolid fermentation, such as temperature, pH, aeration, agitation, and concentration of nutrient and products are not as simple as those for the homogeneous submerged culture. Because of these difficulties, there have been only a limited number of studies on the quantitative measurement of the growth parameters on semisolid substrate. Since the solubility of oxygen in water is extremely low, oxygen supply is often the limiting factor for cell growth in a submerged fermentation. In a semisolid fermentation, however, a thin water film is formed surrounding the insoluble substrate which makes the diffusion of oxygen into the water faster than in liquid fermentation. Therefore, the oxygen requirement level for semisolid fermentation is expected to be less than for liquid fermentation. In this study we have determined the oxygen demand level of C. utilis grown on semisolid straw by measuring the growth rate of the organism under various levels of oxygen supply.

• Research Organization: USDA, Southern Regional Research Center, New Orleans, LA, 70179
• OSTI ID: 5564571
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 30:5
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
DIFFUSION
FERMENTATION
AERATION
ANIMAL FEEDS
CANDIDA
COMPARATIVE EVALUATIONS
CULTIVATION TECHNIQUES
ENZYMES
FILMS
FOOD
GROWTH
LIQUIDS
MUSHROOMS
NUTRIENTS
OPERATION
OXYGEN
PH VALUE
PRODUCTION
QUANTITY RATIO
RESPIRATION
SOLUBILITY
STRAW
SUBSTRATES
TEMPERATURE CONTROL
BIOCONVERSION
CONTROL
ELEMENTS
FLUIDS
FUNGI
MICROORGANISMS
NONMETALS
PLANTS
YEASTS
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