Antibody to endotoxin core glycolipid reverses reticuloendothelial system depression in an animal model of severe sepsis and surgical injury

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · J. Trauma; (United States)

DOI:https://doi.org/10.1097/00005373-198710000-00012· OSTI ID:5564244

Aldridge, M C; Chadwick, S J; Cheslyn-Curtis, S; Rapson, N; Dudley, H A

To study the effect of severe sepsis on the function of the reticuloendothelial system (RES) we have measured the clearance kinetics and organ distribution of both low-dose technetium tin colloid (TTC) and /sup 75/selenomethionine-labelled E. coli in rabbits 24 hours after either sham laparotomy or appendix devascularization. Sepsis resulted in similar delayed blood clearance and reduced liver (Kupffer cell) uptake of both TTC and E. coli. To investigate the ability of polyclonal antibody to E. coli-J-5 (core glycolipid) to improve RES function in the same model of sepsis, further animals were pretreated with either core glycolipid antibody or control serum (10 ml IV) 2 hours before induction of sepsis. TTC clearance kinetics were determined 24 hours later. Antibody pretreated animals showed: a reduced incidence of bacteremia; normalization of the rate of blood clearance and liver uptake of TTC; and a 'rebound' increase in splenic uptake of TTC. We conclude that antibody to E. coli-J-5 enhances bacterial clearance by the RES.

• Research Organization: St. Mary's Hospital, London, England
• OSTI ID: 5564244
• Journal Information: J. Trauma; (United States), Vol. 27:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
BIOLOGICAL FUNCTIONS
ESCHERICHIA COLI
BLOOD-PLASMA CLEARANCE
TISSUE DISTRIBUTION
RETICULOENDOTHELIAL SYSTEM
DYNAMIC FUNCTION STUDIES
TIN
BACTERIAL DISEASES
BIOLOGICAL MODELS
ENDOTOXINS
GLUCOPROTEINS
INJURIES
PERITONITIS
RABBITS
RADIOIMMUNOASSAY
SELENIUM 75
TECHNETIUM
ANIMAL TISSUES
ANIMALS
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM DISEASES
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FUNCTIONS
IMMUNOASSAY
IMMUNOLOGY
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
METALS
MICROORGANISMS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SACCHARIDES
SELENIUM ISOTOPES
TISSUES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques