Proton nuclear magnetic resonance studies of mast cell histamine
Abstract
The state of histamine in mast cells was studied by /sup 1/H NMR spectroscopy. Spectra were measured for histamine in situ in intact mast cells, for histamine in suspensions of mast cell granule matrices that had been stripped of their membranes, and for histamine in solutions of heparin. The /sup 1/H NMR spectrum of intact mast cells is relatively simple, consisting predominantly of resonances for intracellular histamine superimposed on a weaker background of resonances from heparin and proteins of the cells. All of the intracellular histamine contributes of the NMR signals, indicating it must be relatively mobile and not rigidly associated with the negatively charged granule matrix. Spectra for intracellular histamine and for histamine in granule matrices are similar, indicating the latter to be a reasonable model for the in situ situation. The dynamics of binding of histamine by granule matrices and by heparin are considerably different; exchange of histamine between the bulk water and the granule matrices is slow on the /sup 1/H NMR time scale, whereas exchange between the free and bound forms in heparin solution is fast. The chemical shifts of resonances for histamine in mast cells are pH dependent, decreasing as the intragranule pH increases withoutmore »
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Alberta, Edmonton
- OSTI Identifier:
- 5560245
- Resource Type:
- Journal Article
- Journal Name:
- Biochemistry; (United States)
- Additional Journal Information:
- Journal Volume: 26:22
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 62 RADIOLOGY AND NUCLEAR MEDICINE; HISTAMINE; NUCLEAR MAGNETIC RESONANCE; CHEMICAL SHIFT; HEAVY WATER; MAST CELLS; NMR SPECTRA; PH VALUE; PROTONS; AMINES; ANIMAL CELLS; AZOLES; BARYONS; CONNECTIVE TISSUE CELLS; ELEMENTARY PARTICLES; FERMIONS; HADRONS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; IMIDAZOLES; MAGNETIC RESONANCE; NUCLEONS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXYGEN COMPOUNDS; RESONANCE; SOMATIC CELLS; SPECTRA; WATER; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
Citation Formats
Rabenstein, D L, Ludowyke, R, and Lagunoff, D. Proton nuclear magnetic resonance studies of mast cell histamine. United States: N. p., 1987.
Web. doi:10.1021/bi00396a010.
Rabenstein, D L, Ludowyke, R, & Lagunoff, D. Proton nuclear magnetic resonance studies of mast cell histamine. United States. https://doi.org/10.1021/bi00396a010
Rabenstein, D L, Ludowyke, R, and Lagunoff, D. 1987.
"Proton nuclear magnetic resonance studies of mast cell histamine". United States. https://doi.org/10.1021/bi00396a010.
@article{osti_5560245,
title = {Proton nuclear magnetic resonance studies of mast cell histamine},
author = {Rabenstein, D L and Ludowyke, R and Lagunoff, D},
abstractNote = {The state of histamine in mast cells was studied by /sup 1/H NMR spectroscopy. Spectra were measured for histamine in situ in intact mast cells, for histamine in suspensions of mast cell granule matrices that had been stripped of their membranes, and for histamine in solutions of heparin. The /sup 1/H NMR spectrum of intact mast cells is relatively simple, consisting predominantly of resonances for intracellular histamine superimposed on a weaker background of resonances from heparin and proteins of the cells. All of the intracellular histamine contributes of the NMR signals, indicating it must be relatively mobile and not rigidly associated with the negatively charged granule matrix. Spectra for intracellular histamine and for histamine in granule matrices are similar, indicating the latter to be a reasonable model for the in situ situation. The dynamics of binding of histamine by granule matrices and by heparin are considerably different; exchange of histamine between the bulk water and the granule matrices is slow on the /sup 1/H NMR time scale, whereas exchange between the free and bound forms in heparin solution is fast. The chemical shifts of resonances for histamine in mast cells are pH dependent, decreasing as the intragranule pH increases without splitting or broadening. The results are interpreted to indicate that histamine in mast cells is relatively labile, with rapid exchange between histamine and pools of free histamine in water compartments confined in the granule matrix.},
doi = {10.1021/bi00396a010},
url = {https://www.osti.gov/biblio/5560245},
journal = {Biochemistry; (United States)},
number = ,
volume = 26:22,
place = {United States},
year = {Tue Nov 03 00:00:00 EST 1987},
month = {Tue Nov 03 00:00:00 EST 1987}
}