Protein kinase A dependent membrane protein phosphorylation and chloride conductance in endosomal vesicles from kidney cortex

Journal Article · Tue Jan 14 00:00:00 EST 1992 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00116a026· OSTI ID:5560038

Reenstra, W W; Bae, H R; Verkman, A S ^[1]; Sabolic, I ^[2]

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1. Children's Hospital, Oakland, CA (United States) Univ. of California, San Francisco (United States)
2. Massachusetts General Hospital, Charlestown (United States) Harvard Medical School, Charlestown, MA (United States)

Regulation of Cl conductance by protein kinase A action, cell-free measurements of Cl transport and membrane protein phosphorylation were carried out in apical endocytic vesicles from rabbit kidney proximal tubule. Cl transport was measured by a stopped-flow quenching assay in endosomes labeled in vivo with the fluorescent Cl indicator 6-methoxy-N-(3-sulfopropyl)quinolinium. Phosphorylation was studied in a purified endosomal preparation by SDS-PAGE and autoradiography of membrane proteins labeled by ({gamma}-{sup 32}P)ATP. These results suggest that, in a cell-free system, protein kinase A increases Cl conductance in endosomes from kidney proximal tubule by a phosphorylation mechanism. The labeled protein has a size similar to that of the 64-kDa putative kidney Cl channel reported by Landry et al. but is much smaller than the {approximately}170-kDa cystic fibrosis transmembrane conductance regulatory protein.

• OSTI ID: 5560038
• Journal Information: Biochemistry; (United States), Vol. 31:1; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
KIDNEYS
AUTORADIOGRAPHY
MEMBRANE PROTEINS
PHOSPHORYLATION
PHOSPHOTRANSFERASES
BIOCHEMISTRY
CHLORIDES
FLUORESCENCE
PHOSPHORUS 32
QUINOLINES
RABBITS
TUBULES
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHEMICAL REACTIONS
CHEMISTRY
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
ENZYMES
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LUMINESCENCE
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
PYRIDINES
RADIOISOTOPES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques