Amide proton exchange in the. cap alpha. -amylase polypeptide inhibitor tendamistat studied by two-dimensional /sup 1/H nuclear magnetic resonance

Journal Article · Tue Oct 06 00:00:00 EDT 1987 · Biochemistry; (United States)

OSTI ID:5559999

Wang, O; Kline, A D; Wuethrich, K

The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50/sup 0/C were measured by using two-dimensional ..cap alpha..H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the ..beta..-sheet structures. The slowly exchanging protons in the core of the two ..beta..-sheets were shown to exchange via an EX2 mechanism. Further analysis of the data indicates that different large-scale structure fluctuations are responsible for the exchange from the two ..beta..-sheets, even though the three-dimensional structure of Tendamistat appears to consist of a single structural domain.

• Research Organization: Eidgenoessische Technische Hochschule Zuerich-Hoenggerberg, Switzerland
• OSTI ID: 5559999
• Journal Information: Biochemistry; (United States), Vol. 26:20
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ENZYME INHIBITORS
NUCLEAR MAGNETIC RESONANCE
PROTONS
ISOTOPIC EXCHANGE
AMYLASE
CHEMICAL SHIFT
HEAVY WATER
NMR SPECTRA
OVERHAUSER EFFECT
POLYPEPTIDES
BARYONS
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
GLYCOSYL HYDROLASES
HADRONS
HYDROGEN COMPOUNDS
HYDROLASES
MAGNETIC RESONANCE
NUCLEONS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDES
PROTEINS
RESONANCE
SPECTRA
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics