Yeast artificial chromosomes spanning 8 megabases and 10-15 centimorgans of human cytogenetic band Xq26

Journal Article · Wed Jan 01 00:00:00 EST 1992 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.89.1.177· OSTI ID:5559593

Little, R D; Pilia, G; Johnson, S; Schlessinger, D ^[1]; D'Urso, M ^[2]

---

1. Washington Univ., St. Louis, MO (United States)
2. International Inst. of Genetics and Biophysics, Naples (Italy)

A successful test is reported to generate long range contiguous coverage of DNA from a human cytogenetic band in overlapping yeast artificial chromosomes (YACs). Seed YACs in band Xq26 were recovered from a targeted library of clones from Xq24-q28 with 14 probes, including probes for the hypoxanthine guanine phosphoribosyltransferase- and coagulation factor IX-encoding genes and nine probes used in linkage mapping. Neighboring YACs were then identified by 25 walking' steps with end-clones, and the content of 71 probes in cognate YACs was verified by further hybridization analyses. The resultant contig extends across 8 million base pairs, including most of band Xq26, with an order of markers consistent with linkage data. YAC-based mapping, thus, permits steps toward a fully integrated physical and genetic map and is probably adequate to sustain most of the human genome project.

• OSTI ID: 5559593
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 89:1; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HETEROCHROMOSOMES
GENETIC MAPPING
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
DNA SEQUENCING
DNA
DNA HYBRIDIZATION
MAN
ANIMALS
CHROMOSOMES
ENZYMES
GLYCOSYL TRANSFERASES
HYBRIDIZATION
MAMMALS
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PRIMATES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques