Characterization of sheep lung lymph lipoproteins: chemical and physical properties
The authors have determined the composition and distribution of plasma and lung lymph lipoproteins from unanesthetized ewes. Cholesterol, triglyceride, and phospholipid levels in lung lymph were 45%, 50%, and 50%, respectively, of those in plasma. Lipoproteins from both lymph and plasma were separated into two major fractions: d < 1.063 g/ml or LDL, and d 1.063-1.21 g/ml or HDL. HDL was the major lipoprotein species in the plasma and lymph. Gradients gel electrophoresis of HDL on 4-30% gels showed that, in lymph, HDL particles were shifted to larger sizes; in addition to a peak at 8.5 nm, which was similar to plasma HDL, there were two additional components of larger size, one at 9.2 nm and the other at 12 nm. Electron microscopy revealed that lymph HDL contained two new particles not seen in plasma: large, round particles, 13.6 nm diameter, and discoidal particles, 18.7 by 4.9 nm, long and short axis, respectively. Compositional analysis of lymph HDL revealed a relative enrichment in free cholesterol as well as an enrichment in apolipoprotein E. Lymph LDL on gradient gel electrophoresis was extremely heterogeneous. Several peaks were evident in the 23-30 nm size range (similar to plasma LDL), but a supplementary component at approximately 15-16 nm was also present. Whereas plasma LDL on electron microscopy contained only round particles 26 nm in diameter, lymph contained an additional, unusual particle which was close-packed, with square geometry, and was 15 nm in diameter. Changes in the physical and chemical properties of lung lymph lipoproteins suggest that these particles are metabolically modified.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 5558631
- Journal Information:
- J. Lipid Res.; (United States), Vol. 24
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPOPROTEINS
CHEMICAL PROPERTIES
PHYSICAL PROPERTIES
BIOCHEMISTRY
BLOOD PLASMA
CHOLESTEROL
ELECTRON MICROSCOPY
ELECTROPHORESIS
EXPERIMENTAL DATA
LUNGS
LYMPH
PHOSPHOLIPIDS
SHEEP
TRIGLYCERIDES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CHEMISTRY
DATA
DOMESTIC ANIMALS
ESTERS
HYDROXY COMPOUNDS
INFORMATION
LIPIDS
MAMMALS
MATERIALS
MICROSCOPY
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PROTEINS
RESPIRATORY SYSTEM
RUMINANTS
STEROIDS
STEROLS
VERTEBRATES
550200* - Biochemistry