Modified radiometric assay for 3-hydroxy-3-methylglutaryl coenzyme a reductase
A radiometric assay for measuring the activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is described. The assay is based on the separation of the mevalonate product from HMG-CoA by high-voltage electrophoresis. This method is more sensitive and more specific than the NADPH-based spectrophotometric assay, and less tedious than available radiometric assays. It has been used to measure HMG-CoA reductase activity in crude extracts of Saccharomyces cerevisiae and in human skin fibroblasts.
- Research Organization:
- Squibb Institute for Medical Research, Princeton, NJ
- OSTI ID:
- 5558435
- Journal Information:
- Lipids; (United States), Vol. 19:12
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
OXIDOREDUCTASES
ENZYME ACTIVITY
RADIOMETRIC ANALYSIS
CELL CULTURES
ELECTROPHORESIS
FIBROBLASTS
SACCHAROMYCES CEREVISIAE
SKIN
ANIMAL CELLS
BODY
CHEMICAL ANALYSIS
CONNECTIVE TISSUE CELLS
ENZYMES
FUNGI
MICROORGANISMS
ORGANS
PLANTS
QUANTITATIVE CHEMICAL ANALYSIS
SACCHAROMYCES
SOMATIC CELLS
YEASTS
550201* - Biochemistry- Tracer Techniques
OXIDOREDUCTASES
ENZYME ACTIVITY
RADIOMETRIC ANALYSIS
CELL CULTURES
ELECTROPHORESIS
FIBROBLASTS
SACCHAROMYCES CEREVISIAE
SKIN
ANIMAL CELLS
BODY
CHEMICAL ANALYSIS
CONNECTIVE TISSUE CELLS
ENZYMES
FUNGI
MICROORGANISMS
ORGANS
PLANTS
QUANTITATIVE CHEMICAL ANALYSIS
SACCHAROMYCES
SOMATIC CELLS
YEASTS
550201* - Biochemistry- Tracer Techniques