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Title: Animal model for evaluation of topical photoprotection against ultraviolet A (320-380 nm) radiation

Abstract

Recent studies reporting UVA (ultraviolet A radiation 320-380 nm) as an integral part of the cumulative sun-induced damage in human skin have prompted an interest in developing effective UVA photoprotective agents. The development of such compounds has been impeded by the absence of a clinically relevant animal model for evaluating their efficacy. This report describes the development and use of such a laboratory animal system. Selected concentrations of oxybenzone (2-hydroxy-4-methoxybenzophenone) in vehicle (0.1% to 6%) or vehicle alone were applied to the depilated dorsal skin of 30 Hartley strain female albino guinea pigs. The skin was irradiated with solar simulated UVA from a xenon light source. Acute radiation-induced damage was assayed by erythema grading and inhibition of (/sup 3/H)thymidine incorporation into epidermal DNA. Data from erythema grading studies indicated that a significant degree of photoprotection was achieved with 6%, 3%, and 1% solutions of benzophenone compared with the control vehicle; the 6% solution was significantly more photoprotective than the 3% and 1% solutions. A 6% solution afforded significant photoprotection when assayed by (/sup 3/H)thymidine incorporation.

Authors:
; ;
Publication Date:
Research Org.:
Columbia Univ., New York, NY
OSTI Identifier:
5556874
Resource Type:
Journal Article
Journal Name:
J. Invest. Dermatol.; (United States)
Additional Journal Information:
Journal Volume: 89:4
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; RADIOPROTECTIVE SUBSTANCES; EVALUATION; SKIN; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL MODELS; DNA; DNA REPLICATION; DOSE-RESPONSE RELATIONSHIPS; ERYTHEMA; GUINEA PIGS; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ULTRAVIOLET RADIATION; ANIMALS; AZINES; BIOLOGICAL EFFECTS; BODY; DRUGS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; NUCLEIC ACID REPLICATION; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RIBOSIDES; RODENTS; SYMPTOMS; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals; 550201 - Biochemistry- Tracer Techniques

Citation Formats

Chew, S, DeLeo, V A, and Harber, L C. Animal model for evaluation of topical photoprotection against ultraviolet A (320-380 nm) radiation. United States: N. p., 1987. Web. doi:10.1111/1523-1747.ep12471776.
Chew, S, DeLeo, V A, & Harber, L C. Animal model for evaluation of topical photoprotection against ultraviolet A (320-380 nm) radiation. United States. https://doi.org/10.1111/1523-1747.ep12471776
Chew, S, DeLeo, V A, and Harber, L C. 1987. "Animal model for evaluation of topical photoprotection against ultraviolet A (320-380 nm) radiation". United States. https://doi.org/10.1111/1523-1747.ep12471776.
@article{osti_5556874,
title = {Animal model for evaluation of topical photoprotection against ultraviolet A (320-380 nm) radiation},
author = {Chew, S and DeLeo, V A and Harber, L C},
abstractNote = {Recent studies reporting UVA (ultraviolet A radiation 320-380 nm) as an integral part of the cumulative sun-induced damage in human skin have prompted an interest in developing effective UVA photoprotective agents. The development of such compounds has been impeded by the absence of a clinically relevant animal model for evaluating their efficacy. This report describes the development and use of such a laboratory animal system. Selected concentrations of oxybenzone (2-hydroxy-4-methoxybenzophenone) in vehicle (0.1% to 6%) or vehicle alone were applied to the depilated dorsal skin of 30 Hartley strain female albino guinea pigs. The skin was irradiated with solar simulated UVA from a xenon light source. Acute radiation-induced damage was assayed by erythema grading and inhibition of (/sup 3/H)thymidine incorporation into epidermal DNA. Data from erythema grading studies indicated that a significant degree of photoprotection was achieved with 6%, 3%, and 1% solutions of benzophenone compared with the control vehicle; the 6% solution was significantly more photoprotective than the 3% and 1% solutions. A 6% solution afforded significant photoprotection when assayed by (/sup 3/H)thymidine incorporation.},
doi = {10.1111/1523-1747.ep12471776},
url = {https://www.osti.gov/biblio/5556874}, journal = {J. Invest. Dermatol.; (United States)},
number = ,
volume = 89:4,
place = {United States},
year = {Thu Oct 01 00:00:00 EDT 1987},
month = {Thu Oct 01 00:00:00 EDT 1987}
}