Estimation by radiation inactivation of the size of functional units governing Sendai and influenza virus fusion

Journal Article · Tue Sep 22 00:00:00 EDT 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00393a040· OSTI ID:5554536

Bundo-Morita, K; Gibson, S; Lenard, J

The target sizes associated with fusion and hemolysis carried out by Sendai virus envelope glycoproteins were determined by radiation inactivation analysis. The target size for influenza virus mediated fusion with erythrocyte ghosts at pH 5.0 was also determined for comparison. Sendai-mediated fusion with erythrocyte ghosts at pH 7.0 was likewise inactivated exponentially with increasing radiation dose, yielding a target size of 60 +/- 6 kDa, a value consistent with the molecular weight of a single F-protein molecule. The inactivation curve for Sendai-mediated fusion with cardiolipin liposomes at pH 7.0, however, was more complex. Assuming a multiple target-single hit model, the target consisted of 2-3 units of ca. 60 kDa each. A similar target was seen if the liposome contained 10% gangliosides or if the reaction was measured at pH 5.0, suggesting that fusion occurred by the same mechanism at high and low pH. A target size of 261 +/- 48 kDa was found for Sendai-induced hemolysis, in contrast with influenza, which had a more complex target size for this activity. Sendai virus fusion thus occurs by different mechanisms depending upon the nature of the target membrane, since it is mediated by different functional units. Hemolysis is mediated by a functional unit different from that associated with erythrocyte ghost fusion or with cardiolipin liposome fusion.

• Research Organization: UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ
• OSTI ID: 5554536
• Journal Information: Biochemistry; (United States), Vol. 26:19
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HEMOLYSIS
INACTIVATION
VIRUSES
BIOLOGICAL RADIATION EFFECTS
ERYTHROCYTES
GLUCOPROTEINS
INFLUENZA VIRUSES
IONIZING RADIATIONS
SURVIVAL CURVES
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BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
LYSIS
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PATHOLOGICAL CHANGES
PROTEINS
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
560130* - Radiation Effects on Microorganisms