Oxidative metabolites of diethylstilbestrol in the fetal Syrian golden hamster
/sup 14/C-Diethylstilbestrol was administered orally, intraperitoneally, and intrafetally to 15-day pregnant hamsters at a dose of 20 mg/kg body weight, and the radioactivity was determined in the fetus, placenta, and maternal liver after 6 hours. Significant amounts of radioactivity were found in these tissues in every case, indicating maternal-fetal and fetal-maternal transfer of diethylstilbestrol. Part of the radioactivity found in the tissues could not be extracted even after excessive washing. This implied the presence of reactive metabolites. In the fetal and placental extracts, eight oxidative metabolites of diethylstilbestrol were identified by mass fragmentography as hydroxy- and methoxy-derivatives of diethylstilbestrol, pseudodiethylstilbestrol, and dienestrol. The presence of oxidative metabolites in the hamster fetus and the covalent binding to tissue macromolecules are possibly associated with the fetotoxic effects of diethylstilbestrol.
- Research Organization:
- Univ. of Wuerzburg, Federal Republic of Germany
- OSTI ID:
- 5552173
- Journal Information:
- Teratology; (United States), Vol. 30:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cadmium-induced teratogenicity: Association with ROS-mediated endoplasmic reticulum stress in placenta
Autoradiography in fetal golden hamsters treated with tritiated diethylnitrosamine
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
STILBESTROL
TISSUE DISTRIBUTION
TOXICITY
CARBON 14 COMPOUNDS
CHEMICAL BONDS
COVALENCE
FETUSES
HAMSTERS
LIVER
MEMBRANE TRANSPORT
METABOLITES
PLACENTA
PREGNANCY
TRACER TECHNIQUES
ANIMALS
AROMATICS
BODY
DIGESTIVE SYSTEM
DISTRIBUTION
FETAL MEMBRANES
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550501 - Metabolism- Tracer Techniques