Iron uptake by human reticulocytes at physiologic and sub-physiologic concentrations of iron transferrin: The effect of interaction with aluminum transferrin

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · Blood (Journal of Hematology); (United States)

OSTI ID:5552165

Cochran, M; Chawtur, V; Jones, M E; Marshall, E A ^[1]

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1. School of Medicine, Flinders University of South Australia, Bedford Park (Australia)

The authors have studied the interaction, in vitro, between diferric transferrin (FeTr), aluminum transferrin (AlTr), and human reticulocytes harvested from human placental blood. In particular, we aimed to determine the extent to which the kinetics of AlTr and FeTr differed. Using transferrin labeled with either 59Fe or 125I, the association of radiotracer with reticulocytes, as a function both of time and of transferrin concentration, was examined. Under the conditions of the experiments, the data are consistent with a mechanism involving at least three processes. Two early processes acting in parallel behave as a high-affinity saturable receptor and a low-affinity non-saturable receptor, neither of which distinguish between AlTr and FeTr. In a subsequent process, AlTr and FeTr exhibit different kinetics. This third process may be saturated by FeTr but not by AlTr. Interpreted in terms of a current conventional view of metallo-transferrin uptake, we conjecture that the early parallel processes involve cell surface phenomena including classical transferrin-receptor binding, and that the subsequent process represents events, possibly intracellular, involved in metallo-transferrin dissociation or further iron transport. The extent to which AlTr influences the interaction of FeTr with reticulocytes offers insight into both the normal physiology of iron uptake and the potential for toxicity by aluminum.

• OSTI ID: 5552165
• Journal Information: Blood (Journal of Hematology); (United States), Vol. 77:11; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ALUMINIUM
TOXICITY
IRON
MEMBRANE TRANSPORT
TRANSFERRIN
BIOCHEMICAL REACTION KINETICS
BLOOD
DOSE-RESPONSE RELATIONSHIPS
FETUSES
IN VITRO
IODINE 125
IRON 59
PREGNANCY
RECEPTORS
RETICULOCYTES
TIME DEPENDENCE
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ERYTHROCYTES
EVEN-ODD NUCLEI
GLOBULINS
GLOBULINS-BETA
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MATERIALS
MEMBRANE PROTEINS
METALLOPROTEINS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
TRANSITION ELEMENTS
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques