Asynchronous DNA replication within the human. beta. -globin gene locus
- Fred Hutchinson Cancer Research Center, Seattle, WA (USA)
The timing of DNA replication of the human {beta}-globin gene locus has been studied by blot hybridization of newly synthesized BrdUrd-substituted DNA from cells in different stages of the S phase. Using probes that span >120 kilobases across the human {beta}-globin gene locus, the authors show that the majority of this domain replicates in early S phase in the human erythroleukemia cell line K562 and in middle-to-late S phase in the lymphoid cell line Manca. However, in K562 cells three small regions display a strikingly different replication pattern than adjacent sequences. These islands, located in the inter-{gamma}-globin gene region and approximately 20 kilobases 5' to the {epsilon}-globin gene and 20 kilobases 3' to the {beta}-globin gene, replicate later and throughout S phase. A similar area is also present in the {alpha}-globin gene region in K562 cells. They suggest that these regions may represent sites of termination of replication forks.
- OSTI ID:
- 5547080
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:21; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA REPLICATION
MOLECULAR BIOLOGY
GENES
HYBRIDIZATION
CHROMATIN
ERYTHROPOIESIS
GLOBIN
MAN
MITOSIS
PHOSPHORUS 32
TRANSCRIPTION
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD FORMATION
CELL DIVISION
DAYS LIVING RADIOISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEIC ACID REPLICATION
ODD-ODD NUCLEI
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques