Evidence for extracellular, but not intracellular, generation of angiotensin II in the rat adrenal zona glomerulosa

Journal Article · Tue Nov 01 00:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.21.8251· OSTI ID:5547039

Urata, H; Khosla, M C; Bumpus, M; Husain, A ^[1]

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1. Research Institute of the Cleveland Clinic Foundation, OH (USA)

Based on the observation that high levels of renin and angiotensin II (Ang II) are found in the adrenal zona glomerulosa (ZG), it has been postulated that Ang II is formed intracellularly by the renin-converting enzyme cascade in this tissue. To test this hypothesis, the authors examined renin-angiotensin system components in subcellular fractions of the rat adrenal ZG. Renin activity and immunoreactive-Ang II (IR-Ang II) were observed in vesicular fractions but were not colocalized. In addition, angiotensinogen, angiotensin I, and converting enzyme were not observed in the renin or IR-Ang II-containing vesicular fractions. These data do not support the hypothesis that Ang II is formed intracellularly within the renin-containing vesicles of the ZG. Rather, since modulatable renin release from adrenal ZG slices was observed and renin activity was found in dense vesicular fractions (33-39% sucrose), it is likely that Ang II formation in the ZG is extracellular and initiated by the release of vesicular renin. In ZG lysomal fractions {sup 125}I-labeled Ang II was degraded to {sup 125}I-labeled des-(Phe{sup 8})Ang II. Since Ang II antibodies do not recognize des-(Phe{sup 8})Ang II, these finding explain why IR-Ang II in the ZG is due predominantly to Ang II and not to its C-terminal immunoreactive fragments.

• OSTI ID: 5547039
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:21; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANGIOTENSIN
METABOLISM
LABELLED COMPOUNDS
RENIN
SUBCELLULAR DISTRIBUTION
ADRENAL GLANDS
ALDOSTERONE
IODINE 125
LIQUID COLUMN CHROMATOGRAPHY
RATS
ADRENAL HORMONES
ALDEHYDES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR AGENTS
CHROMATOGRAPHY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENZYMES
GLANDS
GLOBULINS
HYDROLASES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KETONES
MAMMALS
MINERALOCORTICOIDS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PREGNANES
PROTEINS
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
STEROIDS
VASOCONSTRICTORS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques