Detection and properties of A-factor-binding protein from Streptomyces griseus
- Univ. of Tokyo (Japan)
The optically active form of tritium-labeled A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone), a pleiotropic autoregulator responsible for streptomycin production, streptomycin resistance, and sporulation in Streptomyces griseus, was chemically synthesized. By using the radioactive A-factor, a binding protein for A-factor was detected in the cytoplasmic fraction of this organism. The binding protein had an apparent molecular weight of approximately 26,000, as determined by gel filtration. Scatchard analysis suggested that A-factor bound the protein in the molar ratio of 1:1 with a binding constant, Kd, of 0.7 nM. The number of the binding protein was roughly estimated to be 37 per genome. The inducing material virginiae butanolide C (VB-C), which has a structure very similar to that of A-factor and is essential for virginiamycin production in Streptomyces virginiae, did not inhibit binding. In addition, no protein capable of specifically binding {sup 3}H-labeled VB-C was found in S. griseus. Together with the observation that VB-C had almost no biological activity on the restoration of streptomycin production or sporulation in an A-factor-deficient mutant of S. griseus, these results indicated that the binding protein had a strict ligand specificity. Examination for an A-factor-binding protein in Streptomyces coelicolor A3(2) and Streptomyces lividans showed the absence of any specifically binding protein.
- OSTI ID:
- 5546889
- Journal Information:
- Journal of Bacteriology; (USA), Vol. 171:8; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
CHEMICAL PROPERTIES
BIOCHEMICAL REACTION KINETICS
CHEMICAL PREPARATION
CYTOPLASM
GROWTH FACTORS
MOLECULAR WEIGHT
SPECIFICITY
STREPTOMYCES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BACTERIA
CELL CONSTITUENTS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MICROORGANISMS
MITOGENS
ORGANIC COMPOUNDS
REACTION KINETICS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques