Brain polyphosphoinositide metabolism during focal ischemia in rat cortex

Journal Article · Mon Apr 01 00:00:00 EST 1991 · Stroke; (USA)

DOI:https://doi.org/10.1161/01.STR.22.4.495· OSTI ID:5545736

Lin, T N; Liu, T H; Xu, J; Hsu, C Y; Sun, G Y ^[1]

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1. Univ. of Missouri, Columbia (USA)

Using a rat model of stroke, we examined the effects of focal cerebral ischemia on the metabolism of polyphosphoinositides by injecting {sup 32}Pi into both the left and right cortices. After equilibration of the label for 2-3 hours, ischemia induced a significant decrease (p less than 0.001) in the concentrations of labeled phosphatidyl 4,5-bisphosphates (66-78%) and phosphatidylinositol 4-phosphate (64-67%) in the right middle cerebral artery cortex of four rats. The phospholipid labeling pattern in the left middle cerebral artery cortex, which sustained only mild ischemia and no permanent tissue damage, was not different from that of two sham-operated controls. However, when {sup 32}Pi was injected 1 hour after the ischemic insult, there was a significant decrease (p less than 0.01) in the incorporation of label into the phospholipids in both cortices of four ischemic rats compared with four sham-operated controls. Furthermore, differences in the phospholipid labeling pattern were observed in the left cortex compared with the sham-operated controls. The change in labeling pattern was attributed to the partial reduction in blood flow following ligation of the common carotid arteries. We provide a sensitive procedure for probing the effects of focal cerebral ischemia on the polyphosphoinositide signaling pathway in the brain, which may play an important role in the pathogenesis of tissue injury.

• OSTI ID: 5545736
• Journal Information: Stroke; (USA), Vol. 22:4; ISSN 0039-2499
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
METABOLISM
ISCHEMIA
PATHOGENESIS
PHOSPHOLIPIDS
BIOLOGICAL PATHWAYS
CEREBRAL ARTERIES
CEREBRAL CORTEX
PHOSPHATES
PHOSPHORUS 32
RATS
TRACER TECHNIQUES
ANEMIAS
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CEREBRUM
DAYS LIVING RADIOISOTOPES
DISEASES
ESTERS
HEMIC DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
NERVOUS SYSTEM
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RODENTS
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques