Effect of monoclonal antibodies on limited proteolysis of native glycoprotein gD of herpes simplex virus type 1
- Univ. of Pennsylvania, Philadelphia, PA
We examined the properties of 17 monoclonal antibodies to glycoprotein gD of herpes simplex type 1 (HSV-1) (gD-1) and HSV-2 (gD-2). The antibodies recognized eight separate determinants of gD, based on differences in radioimmuno-precipitation and neutralization assays. The determinants were distributed as follows: three were gD-1 specific, one was gD-2 specific, and four were type common. Several type-specific and type-common determinants appeared to be involved in neutralization. We developed a procedure for examining the effect that binding of monoclonal antibody has on proteolysis of native gD-1 by Staphylococcus aureus protease V8. We showed that several different patterns of protease V8 cleavage were obtained, depending on the monoclonal antibody used. The proteolysis patterns were generally consistent with the immunological groupings. With four groups of antibodies, we found that fragments of gD-1 remained bound to antibody after V8 treatment. A 38,000-dalton fragment remained bound to antibodies in three different groups of monoclonal antibodies. This fragment appeared to contain one type-common and two type-specific determinants. A 12,000-dalton fragment remained bound to antibodies belonging to one type-common group of monoclonal antibodies. Tryptic peptide analysis revealed that the 12,000-dalton fragment represented a portion of the 38,000-dalton fragment and was enriched in a type-common arginine tryptic peptide.
- OSTI ID:
- 5545583
- Journal Information:
- J. Virol.; (United States), Vol. 41:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Molecular epitope identification by limited proteolysis of an immobilized antigen-antibody complex and mass spectrometric peptide mapping
Localization and synthesis of an antigenic determinant of herpes simplex virus glycoprotein D that stimulates the production of neutralizing antibody
Related Subjects
ANTIBODIES
IMMUNE REACTIONS
GLUCOPROTEINS
PROTEOLYSIS
RESPONSE MODIFYING FACTORS
ENZYME ACTIVITY
HERPES SIMPLEX
LABELLED COMPOUNDS
SULFUR 35
SULFUR COMPOUNDS
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DISEASES
EVEN-ODD NUCLEI
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SKIN DISEASES
SULFUR ISOTOPES
VIRAL DISEASES
550201* - Biochemistry- Tracer Techniques