Molecular characterization of haustorial development in Striga asiatica
- Univ. of Virginia, Charlottesville (USA)
S. asiatica is a root parasite of agronomically important monocots. It attaches to and penetrates its host plant roots by the formation of an unique organ termed a haustorium. Host derived substituted-benzoquinones (i.e., 2, 6 -DMBQ) have been shown to induce a developmental switch from radicle elongation to haustorial formation. We have investigated the role of protein synthesis in haustorial development by inhibitor studies and in vivo feedings experiments with {sup 35}S-labelled amino acids. Our results demonstrate that continued protein synthesis is necessary for both the perception of haustorial inducer compounds and for haustorial maturation. Two-dimensional PAGE was used to monitor changes in the profiles of in vivo synthesized proteins during haustorial development. Altered synthetic activity, including the accumulation of haustorial specific proteins, is observed throughout the 18-24 hr time period for haustorial development, with some changes evident as early as 3-6 hr post-induction. Differential screening of {lambda}gt11 expression libraries prepared from 18 hr induced radicles is being used to identify developmentally regulated, haustorial specific gene products.
- OSTI ID:
- 5545571
- Report Number(s):
- CONF-9007196-; CODEN: PPYSA
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
LILIOPSIDA
PARASITIC DISEASES
ONTOGENESIS
MOLECULAR BIOLOGY
PROTEINS
ENZYME INDUCTION
MAGNOLIOPSIDA
PARASITES
SULFUR 35
SULFUR COMPOUNDS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
GENE REGULATION
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
NUCLEI
ORGANIC COMPOUNDS
PLANTS
RADIOISOTOPES
SULFUR ISOTOPES
553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)
090800 - Biomass Fuels- Production- (1990-)
550201 - Biochemistry- Tracer Techniques