Fibronectin receptors of mononuclear phagocytes: Binding characteristics and biochemical isolation

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90099-2· OSTI ID:5533880

Garcia-Pardo, A; Ferreira, O C; Valinsky, J; Bianco, C ^[1]

---

1. New York Blood Center, NY (United States)

Fibronectin receptors on mononuclear phagocytes are involved in the localization of monocytes at inflammatory sites and in the subsequent expression of macrophage-like phenotypes. In this study, the authors have investigated the hypothesis that preteolytically derived fragments of fibronectin may interfere with binding of fibronectin to monocytes in the extracellular matrix. They report on the reactivity of U937 cells with an 80-kDa tryptic fragment of fibronectin which contains the cell-binding domain but lacks the gelatin/collagen-binding domain. U937 cells attached to surfaces coated with the 80-kDa fragment as well as with intact fibronectin. Preincubation of the cells with the 80-kDa fragment inhibited attachment to both surfaces while intact fibronectin had little or no inhibitory effect. This complex resolved into a single diffuse band of 144 kDa upon reduction. Binding of the protein complex to the affinity column required divalent cations. The complex bound to wheat germ agglutinin and could be specifically eluted by N-acetylglucosamine. Similar cell-surface proteins were isolated from peripheral blood monocytes.

• OSTI ID: 5533880
• Journal Information: Experimental Cell Research; (United States), Vol. 181:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FIBRIN
RADIORECEPTOR ASSAY
CATIONS
MONOCYTES
PHAGOCYTES
PRECIPITATION
PURIFICATION
RECEPTORS
TRITIUM COMPOUNDS
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY FLUIDS
CHARGED PARTICLES
COAGULANTS
DRUGS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROGEN COMPOUNDS
IONS
ISOTOPE APPLICATIONS
LEUKOCYTES
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PROTEINS
SCLEROPROTEINS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
550301* - Cytology- Tracer Techniques