Reduction of EGF receptor levels in human tumor cells transfected with an antisense RNA expression vector

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90367-4· OSTI ID:5533771

Yamada, Hirotomo; Koizumi, Shinji; Kimura, Masami ^[1]; Shimizu, Nobuyoshi ^[2]

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1. National Inst. of Industrial Health, Kawasaki (Japan)
2. Keio Univ., Tokyo (Japan)

An expression vector was constructed from part of pSV2neo with the 3{prime}-ClaI fragment of the epidermal growth factor (EGF) receptor cDNA inserted in an inverted orientation downstream from the human metallothionein (MT) IIa promoter. The human squamous carcinoma cell line NA, which overproduces EGF receptor, was transfected with this vector and selected for resistance to the neomycin derivative G418. One of the stable transfectants had a 90% reduction cell-surface EGF receptor in response to ZnSO{sub 4}. The nascent EGF receptor peptide was also decreased with concurrent induction of MT mRNA. These data suggest that the antisense transcript regulated by the MT promoter inhibits the expression of the endogenous EGF receptor genes. Although no transcripts from the antisense gene were detected, the results indicate that transfection with the antisense vector provides a technique by which to modulate the number of EGF receptors on the cell surface of squamous cell carcinomas.

• OSTI ID: 5533771
• Journal Information: Experimental Cell Research; (United States), Vol. 184:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
RADIORECEPTOR ASSAY
RECEPTORS
GENE REGULATION
TUMOR CELLS
GENE RECOMBINATION
EPIDERMIS
GENES
IODINE 125
METALLOTHIONEIN
NEOMYCIN
ZINC SULFATES
ANIMAL CELLS
ANIMAL TISSUES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANE PROTEINS
METALLOPROTEINS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
SKIN
SULFATES
SULFUR COMPOUNDS
TISSUES
TRACER TECHNIQUES
ZINC COMPOUNDS
550301* - Cytology- Tracer Techniques