Patterns of (3H) thymidine incorporation differ in immature rats and mature, cycling rats
By the time follicular development has progressed to the preovulatory stage, granulosa cells abutting the basement membrane no longer incorporate (3H) thymidine (3H-TdR). The purpose of this experiment was to determine when, during the course of follicular growth, cell proliferation in these mural granulosa cells ceases. Autoradiographs were prepared following continuous 3H-TdR infusion in vivo, or incubation with 3H-TdR in vitro. In cycling rats, the concentration of silver grains over mural regions of the granulosa layer was lower than over antral regions of most follicles with greater than 1000 cells in the largest cross section (LCS). This centripetal labeling pattern became more striking as follicular size increased. By proestrus, only the cells of the discus proligerus (cumulus and the portion of the follicular wall supporting the cumulus oocyte complex) continued to incorporate 3H-TdR. In contrast to cycling rats, centripetal labeling patterns were not seen in ovaries of prepubertal rats, even in follicles of the same size. The difference in follicular growth patterns between these two types of animals suggests an influence of cyclic gonadotropin surges on the control of granulosa cell proliferation.
- Research Organization:
- Univ. of Maryland School of Medicine, Baltimore
- OSTI ID:
- 5531871
- Journal Information:
- Biol. Reprod.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
OVULATION
BIOCHEMISTRY
THYMIDINE
UPTAKE
TRITIUM COMPOUNDS
AGE DEPENDENCE
AUTORADIOGRAPHY
CELL PROLIFERATION
DNA REPLICATION
GONADOTROPINS
RATS
STEM CELLS
ANIMAL CELLS
ANIMALS
AZINES
CHEMISTRY
HETEROCYCLIC COMPOUNDS
HORMONES
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques