Application of /sup 31/P-NMR spectroscopy to the study of striated muscle metabolism
Abstract
This review presents the principles and limitations of phosphorus nuclear magnetic resonance (/sup 31/P-NMR) spectroscopy as applied to the study of striated muscle metabolism. Application of the techniques discussed include noninvasive measurement of high-energy phosphate, intracellular pH, intracellular free Mg/sup 2 +/, and metabolite compartmentation. In perfused cat biceps (fast-twitch) muscles, but not in soleus (slow-twitch), NMR spectra indicate a substantially lower (1 mM) free inorganic phosphate level than when measured chemically (6 mM). In addition, saturation and inversion spin-transfer methods that enable direct measurement of the unidirectional fluxes through creatine kinase are described. In perfused cat biceps muscle, results suggest that this enzyme and its substrates are in simple chemical equilibrium.
- Authors:
- Publication Date:
- OSTI Identifier:
- 5530091
- Resource Type:
- Journal Article
- Journal Name:
- Am. J. Physiol.; (United States)
- Additional Journal Information:
- Journal Volume: 242:1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; MUSCLES; METABOLISM; NUCLEAR MAGNETIC RESONANCE; REVIEWS; CATS; ENZYMES; MAGNESIUM; METABOLITES; NMR SPECTRA; PH VALUE; PHOSPHATES; PHOSPHORUS 31; PHYSIOLOGY; ALKALINE EARTH METALS; ANIMALS; DOCUMENT TYPES; ELEMENTS; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; METALS; NUCLEI; ODD-EVEN NUCLEI; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; RESONANCE; SPECTRA; STABLE ISOTOPES; VERTEBRATES; 550501* - Metabolism- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques
Citation Formats
Meyer, R A, Kushmerick, M J, and Brown, T R. Application of /sup 31/P-NMR spectroscopy to the study of striated muscle metabolism. United States: N. p., 1982.
Web.
Meyer, R A, Kushmerick, M J, & Brown, T R. Application of /sup 31/P-NMR spectroscopy to the study of striated muscle metabolism. United States.
Meyer, R A, Kushmerick, M J, and Brown, T R. 1982.
"Application of /sup 31/P-NMR spectroscopy to the study of striated muscle metabolism". United States.
@article{osti_5530091,
title = {Application of /sup 31/P-NMR spectroscopy to the study of striated muscle metabolism},
author = {Meyer, R A and Kushmerick, M J and Brown, T R},
abstractNote = {This review presents the principles and limitations of phosphorus nuclear magnetic resonance (/sup 31/P-NMR) spectroscopy as applied to the study of striated muscle metabolism. Application of the techniques discussed include noninvasive measurement of high-energy phosphate, intracellular pH, intracellular free Mg/sup 2 +/, and metabolite compartmentation. In perfused cat biceps (fast-twitch) muscles, but not in soleus (slow-twitch), NMR spectra indicate a substantially lower (1 mM) free inorganic phosphate level than when measured chemically (6 mM). In addition, saturation and inversion spin-transfer methods that enable direct measurement of the unidirectional fluxes through creatine kinase are described. In perfused cat biceps muscle, results suggest that this enzyme and its substrates are in simple chemical equilibrium.},
doi = {},
url = {https://www.osti.gov/biblio/5530091},
journal = {Am. J. Physiol.; (United States)},
number = ,
volume = 242:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}