Role of liver endothelium in the binding and uptake of ceruloplasmin: studies with colloidal gold probe

Journal Article · Fri Feb 01 00:00:00 EST 1985 · J. Ultrastruct. Res.; (United States)

DOI:https://doi.org/10.1016/0889-1605(85)90109-0· OSTI ID:5529133

Kataoka, M; Tavassoli, M

To determine the mode of uptake of ceruloplasmin (CP) by liver, the protein was labeled with colloidal gold and infused into the portal vein. In cold almost all probes bound to the sinusoidal endothelium, and at 37/sup 0/C internalization via a system of coated pits and vesicles occurred. Only rarely did the probe appear to bypass the endothelium, moving to the albuminal side through the gaps between endothelial cells. In the endothelial cytoplasm, the probe was seen in coated vesicles, endosomes, tubules, and large vesicles which may have formed by fusion of endosomes and tubules. Moreover, externalization of the probe to the abluminal side was noted, and this also occurred via a system of coated vesicles. The findings suggest that the uptake of CP in the liver may be primarily a transendothelial phenomenon (transcytosis).

• OSTI ID: 5529133
• Journal Information: J. Ultrastruct. Res.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CERULOPLASMIN
UPTAKE
GOLD ISOTOPES
CHEMICAL BONDS
ENDOTHELIUM
LIVER
RADIOCOLLOIDS
RATS
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BODY
COLLOIDS
COMPLEXES
COPPER COMPLEXES
DIGESTIVE SYSTEM
DISPERSIONS
GLANDS
GLOBULINS
GLOBULINS-ALPHA
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALLOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
TISSUES
TRANSITION ELEMENT COMPLEXES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques