skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: pLS101 plasmid vector

Patent ·
OSTI ID:5528773

Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb ma1M gene fragment ligated to a 4.4 Kb Tcr DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems. 5 figs., 2 tabs.

Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
DOE Contract Number:
AC02-76CH00016
Assignee:
Dept. of Energy
Application Number:
ON: DE86013777
OSTI ID:
5528773
Resource Relation:
Other Information: Paper copy only, copy does not permit microfiche production
Country of Publication:
United States
Language:
English