Assessment of the role of oxygen and mitochondria in heat shock induction of radiation and thermal resistance in Saccharomyces cerevisiae
In response to a heat shock, the yeast Saccharomyces cerevisiae undergoes a large increase in its resistance to heat and, by the induction of its recombinational DNA repair capacity, a corresponding increase in resistance to radiation. Yeast which lack mitochondrial DNA, mitochondria-controlled protein synthetic apparatus, aerobic respiration, and electron transport (rho/sup 0/ strain) were used to assess the role of O/sub 2/, mitochondria, and oxidative processes controlled by mitochondria in the induction of these resistances. We have found that rho/sup 0/ yeast grown and heat shocked in either the presence or absence of O/sub 2/ are capable of developing both radiation and heat resistance. We conclude that neither the stress signal nor its cellular consequences of induced heat and radiation resistance are directly dependent on O/sub 2/, mitochondrial DNA, or mitochondria-controlled protein synthetic or oxidative processes.
- Research Organization:
- Atomic Energy of Canada Ltd., Chalk River, Ontario
- OSTI ID:
- 5527605
- Journal Information:
- Radiat. Res.; (United States), Vol. 96:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
HEAT
RADIOSENSITIVITY EFFECTS
MITOCHONDRIA
BIOCHEMISTRY
OXYGEN
PROTEINS
BIOSYNTHESIS
BIOLOGICAL REPAIR
SACCHAROMYCES CEREVISIAE
X RADIATION
BIOLOGICAL RECOVERY
CELL CONSTITUENTS
CHEMISTRY
ELECTROMAGNETIC RADIATION
ELEMENTS
ENERGY
FUNGI
IONIZING RADIATIONS
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
ORGANOIDS
PLANTS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
SYNTHESIS
YEASTS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
550700 - Microbiology
550200 - Biochemistry