Effect of dietary fat levels on the susceptibility of colonic cells to nuclear-damaging agents
The effect of two levels and types of dietary fats on the susceptibility of colonic cells to the nuclear-damaging effect of 1,2-dimethylhydrazine dihydrochloride (DMH), 2-amino-3,4-dimethylimidazo(4,5-f)quinoline (MeIQ), and gamma-radiation was investigated. Corn oil and beef tallow were added to the semisynthetic diet at 5% and 20% levels (weight/weight). A diet-related effect was not evident until after two weeks of feeding. Animals (C57BL/6J female mice) that were given the 20% corn oil or beef tallow diets had significantly (p less than 0.05) more nuclear aberrations in their colons 24 hours following treatment with DMH (5 mg or 10 mg/kg body wt or MeIQ (100 mg/kg body weight) than did those given low-fat diets (5% corn oil or beef tallow). The nuclear-damaging effect of gamma radiation was unaffected by dietary treatments. A high-fat diet had the most pronounced effect on DMH-treated animals, and maximum nuclear aberrations were observed 24 hours following the treatment. Thus, we concluded that increased levels of dietary fats elevate the toxicity of DMH and MeIQ to colonic epithelial cells.
- Research Organization:
- Ludwig Institute for Cancer Research, Toronto, Ontario, Canada
- OSTI ID:
- 5525168
- Journal Information:
- Nutr. Cancer; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EPITHELIUM
SENSITIVITY
QUINOLINES
BIOLOGICAL EFFECTS
RADIOSENSITIVITY EFFECTS
CELL NUCLEI
DIET
FATS
GAMMA RADIATION
HYDRAZINE
LARGE INTESTINE
MICE
ANIMAL TISSUES
ANIMALS
AROMATICS
AZAARENES
AZINES
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
INTESTINES
IONIZING RADIATIONS
MAMMALS
NITROGEN COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIDINES
RADIATIONS
RODENTS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals