Induction of heat-shock protein synthesis in chondrocytes at physiological temperatures
Induction of heat-shock protein (HSP) synthesis is demonstrated in cultured calf-chondrocytes at temperatures shown to occur in normal human cartilage during experiments subjecting intact cadaverous hip joints to the parameters of level walking. A 70,000 MW heat-shock protein (HSP-70) is synthesized by chondrocytes at temperatures above 39 degrees C, while induction of synthesis of a 110,000 MW HSP only occurs at temperatures of 45 degrees C or greater. These differences in critical temperatures for induction, and data showing differences in kinetics of induction and repression of synthesis, suggest that there are differences in the mechanism of induction of the two HSPs. The duration of HSP synthesis and inhibition of synthesis of normal cellular proteins is directly proportional to the duration and magnitude of the temperature rise. Possible relationships between these new findings and the initiation and progression of degenerative joint disease are discussed.
- Research Organization:
- Massachusetts Institute of Technology, Cambridge
- OSTI ID:
- 5520616
- Journal Information:
- J. Orth. Res.; (United States), Vol. 3:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
The epigenetic effect of glucosamine and a nuclear factor-kappa B (NF-kB) inhibitor on primary human chondrocytes - Implications for osteoarthritis
Articular chondrocyte metabolism and osteoarthritis
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PROTEINS
BIOSYNTHESIS
TEMPERATURE EFFECTS
ANIMAL CELLS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CARTILAGE
CATTLE
CELL CULTURES
SULFUR ISOTOPES
TRITIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
BODY
CONNECTIVE TISSUE
DOMESTIC ANIMALS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
REACTION KINETICS
RUMINANTS
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
560205 - Thermal Effects- Vertebrates- (-1987)