Analytical techniques for cell fractions
Journal Article
·
· Anal. Biochem.; (United States)
Disposable microimmunoadsorbent columns containing Staphylococcus Protein A and monoclonal antibodies were used to bind antigenic proteins from a mixture. Eluates from these columns were directly analyzed by electrophoresis on two-dimensional (2-D) gels. In this way, biochemical and biophysical information on the bound antigen and on the specific antibody can be obtained simultaneously. The microimmunoadsorbents are easy to handle and in conjunction with multiple 2-D gel systems provide a means for screening large numbers of myeloma hybrids for specificity to antigens in complex mixtures.
- Research Organization:
- Argonne National Lab., IL
- OSTI ID:
- 5520107
- Journal Information:
- Anal. Biochem.; (United States), Vol. 101:2
- Country of Publication:
- United States
- Language:
- English
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