Differentiation and carcinogenesis: An integrated multilevel study of mechanisms from molecules to man

Technical Report · Tue Aug 01 00:00:00 EDT 1989

OSTI ID:5516888

Ts'o, P O.P. ^[1]

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1. Johns Hopkins Univ., Baltimore, MD (USA)

This research proposal consists of four scientific projects and one administrative core. Project I concerns the investigation of the mechanisms regulating normal cellular differentiation and the disruption of these regulatory mechanisms in neoplastic cells, using myogenic differentiation as a model system. Project II concerns three investigations: (1) effects of oxygen radicals and x-ray irradiation; (2) development of computerized microscopic imaging system; and (3) gene location and control of cellular gene expression. Project III concerns the two-dimensional DNA restriction fragment mapping for the E. coli genome (3 megabase in size) and yeast genome (12 megabase in size), as well as the development of a probe-free, global monitoring system for DNA rearrangement in E. coli and in yeast. Project IV provides these analogs for Projects I, II, and III for the purpose of controlling gene expression at both the mRNA level or directly at the DNA level with a sequence-specific triplex formation. All these projects work closely with each other in the study on the localization and organization of genes, damage to genes, and control of gene expression in a fully integrated approach.

• Research Organization: Johns Hopkins Univ., Baltimore, MD (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG02-88ER60636
• OSTI ID: 5516888
• Report Number(s): DOE/ER/60636-2; ON: DE89017522
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARCINOGENESIS
MECHANICS
BACTERIA
CARCINOGENS
CELL CULTURES
CELL DIFFERENTIATION
DNA REPLICATION
GENES
HAMSTERS
MICE
MOLECULAR BIOLOGY
MYOBLASTS
PROGRESS REPORT
STEM CELLS
ANIMAL CELLS
ANIMALS
DOCUMENT TYPES
MAMMALS
MICROORGANISMS
MUSCLES
NUCLEIC ACID REPLICATION
PATHOGENESIS
RODENTS
SOMATIC CELLS
VERTEBRATES
550200* - Biochemistry
550300 - Cytology