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Title: The effect of BAY o 2752 on bile acid absorption and cholesterol esterification

Abstract

BAY o 2752 (N,N-(1,11-undecandiyl)bis(2,3-dihydro-2-methyl-1H-indole-1-carboxamide)) has been demonstrated to inhibit intestinal cholesterol absorption in rats. Studies were carried out on male Wistar rats to determine if this drug alters intestinal bile acid absorption or cholesterol esterification by acyl CoA: cholesterol acyltransferase (ACAT) or cholesterol ester hydrolase (CEH). BAY o 2752 did not affect intestinal absorption of taurocholic acid (TC) from ileal segments perfused in vivo with a tragacanth suspension in phosphate buffer containing NaCl, TC, and 24-{sup 14}C-TC as determined by the excretory rate of radioactivity in bile. BAY o 2752 also did not affect the uptake of TC into ileal everted sacs incubated in stirred, gassed Krebs-Ringer bicarbonate buffer with 1 mM TC, 24-{sup 14}C-TC and {sup 3}H-inulin. BAY o 2752 also did not bind TC; TG, in a filtrate of the above solutions remained at 92-98% of control.

Authors:
Publication Date:
Research Org.:
Boston Univ., MA (USA)
OSTI Identifier:
5516571
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMIDES; BIOCHEMICAL REACTION KINETICS; BILE ACIDS; INTESTINAL ABSORPTION; CHOLESTEROL; ESTERIFICATION; BUFFERS; CARBON 14 COMPOUNDS; ENZYME ACTIVITY; HYDROLASES; INULIN; RATS; TRACER TECHNIQUES; TRANSFERASES; TRITIUM COMPOUNDS; ABSORPTION; ANIMALS; CARBOHYDRATES; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; ENZYMES; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; POLYSACCHARIDES; REACTION KINETICS; RODENTS; SACCHARIDES; STEROIDS; STEROLS; UPTAKE; VERTEBRATES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Harnett, K M. The effect of BAY o 2752 on bile acid absorption and cholesterol esterification. United States: N. p., 1988. Web.
Harnett, K M. The effect of BAY o 2752 on bile acid absorption and cholesterol esterification. United States.
Harnett, K M. 1988. "The effect of BAY o 2752 on bile acid absorption and cholesterol esterification". United States.
@article{osti_5516571,
title = {The effect of BAY o 2752 on bile acid absorption and cholesterol esterification},
author = {Harnett, K M},
abstractNote = {BAY o 2752 (N,N-(1,11-undecandiyl)bis(2,3-dihydro-2-methyl-1H-indole-1-carboxamide)) has been demonstrated to inhibit intestinal cholesterol absorption in rats. Studies were carried out on male Wistar rats to determine if this drug alters intestinal bile acid absorption or cholesterol esterification by acyl CoA: cholesterol acyltransferase (ACAT) or cholesterol ester hydrolase (CEH). BAY o 2752 did not affect intestinal absorption of taurocholic acid (TC) from ileal segments perfused in vivo with a tragacanth suspension in phosphate buffer containing NaCl, TC, and 24-{sup 14}C-TC as determined by the excretory rate of radioactivity in bile. BAY o 2752 also did not affect the uptake of TC into ileal everted sacs incubated in stirred, gassed Krebs-Ringer bicarbonate buffer with 1 mM TC, 24-{sup 14}C-TC and {sup 3}H-inulin. BAY o 2752 also did not bind TC; TG, in a filtrate of the above solutions remained at 92-98% of control.},
doi = {},
url = {https://www.osti.gov/biblio/5516571}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}

Thesis/Dissertation:
Other availability
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