RNA from an immediate early region of the type 1 herpes simplex virus genome is present in the trigeminal ganglia of latently infected mice
Transcription of the type 1 herpes simplex virus (HSV-1) genome in trigeminal ganglia of latently infected mice was studied using in situ hybridization. Probes representative of each temporal gene class were used to determine the regions of the genome that encode the transcripts present in latently infected cells. Probes encoding HSV-1 sequences of the five immediate early genes and representative early (thymidine kinase), early-late (major capsid protein), and late (glycoprotein C) genes were used in these experiments. Of the probes tested, only those encoding the immediate early gene product infected-cell polypeptide (ICP) 0 hybridized to RNA in latently infected tissues. Probes containing the other immediate early genes (ICP4, ICP22, ICP27, and ICP47) and the representative early, early-late, and late genes did not hybridize. Two probes covering approx. = 30% of the HSV-1 genome and encoding over 20 early and late transcripts also did not hybridize to RNA in latently infected tissues. These results, with probes spanning > 60% of the HSV-1 genome, suggest that transcription of the HSV-1 genome is restricted to one region in latently infected mouse trigeminal ganglia.
- Research Organization:
- Wistar Institute, Philadelphia, PA
- OSTI ID:
- 5515078
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:10
- Country of Publication:
- United States
- Language:
- English
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NERVES
AUTORADIOGRAPHY
PROTEINS
HYBRIDIZATION
RNA
GENES
HERPES SIMPLEX
MICE
PLASMIDS
SULFUR 35
TRANSCRIPTION
VIRAL DISEASES
VIRUSES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIOISOTOPES
RODENTS
SKIN DISEASES
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques