Decreased thyroidal response to thyrotropin in diabetic mice

Journal Article · Sun Nov 01 00:00:00 EST 1981 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-109-5-1428· OSTI ID:5513277

Bagchi, N; Brown, T R; Shivers, B; Lucas, S; Mack, R E

The effect of diabetes mellitus on the synthesis and secretion of thyroid hormone ws investigated in mice with streptozotocin-induced diabetes. Thyroid glands were labeled in vivo with 131I for 2 h. In control animals, TSH stimulated the synthesis of PB127I and 131I-labeled iodothyronines and simultaneously decreased the proportion of 131I-. These effects of TSH were not observed in diabetic animals but were demonstrable in diabetic animals treated with insulin. For studies of hormone secretion, labeled thyroid glands were cultured in vitro in medium containing 1 mM mononitrotyrosine. The rate of the hydrolysis of labeled thyroglobulin was measured as the proportion of 131I-labeled iodotyrosines and 131I-labeled iodothyronines recovered at the end of culture and was used as an index of thyroid secretion. TSH in vivo stimulated the rate of thyroglobulin hydrolysis for 6 h, with a peak occurring after 2 h. The diabetic mice had a diminished response to TSH, which improved on treatment with insulin. The addition of TSH and insulin to the culture medium significantly increased the rate of thyroglobulin hydrolysis in glands of diabetic mice over that resulting from the addition of dibutyryl cAMP alone. The generation of thyroidal cAMP in response to TSH was higher in diabetic mice than in controls. The rise in plasma T4 and T3 2 h after the administration of TSH was less in diabetic mice than in control mice or diabetic mice treated with insulin. Our studies, therefore, indicate that the thyroidal response to TSH is decreased in diabetes mellitus. The defect appears to be at a step beyond the generation of cAMP.

• Research Organization: Department of Medicine, Wayne State University, Detroit, MI
• OSTI ID: 5513277
• Journal Information: Endocrinology; (United States), Vol. 109:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DIABETES MELLITUS
PATHOLOGY
IODINE 131
ISOTOPE APPLICATIONS
THYROID HORMONES
BIOCHEMICAL REACTION KINETICS
AMP
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
MICE
THYROGLOBULIN
THYROID
THYRONINE
TSH
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
GLOBULINS
HORMONES
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
METABOLIC DISEASES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SYNTHESIS
VERTEBRATES
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550201 - Biochemistry- Tracer Techniques
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